慢性低氧高二氧化碳对小鼠大脑线粒体功能的影响（临床医学资料）.docVIP

慢性低氧高二氧化碳对小鼠大脑线粒体功能的影响（临床医学资料） 文档信息 属性： F-01NWKA，doc格式，正文30596字。质优实惠，欢迎下载！ 适用： 作为医学资料、临床医学资料写作的参考文献，解决如何写好实用应用文、正确编写文案格式、内容摘取等相关工作。 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：慢性低氧高二氧化碳对小鼠大脑线粒体功能的影响 1 1??材料和方法 3 2??结果 5 3??讨论 6 文2：浅论慢性低氧高二氧化碳对小鼠大脑线粒体功能的影响 7 1材料和方法 9 参考文摘引言： 12 原创性声明（模板） 14 文章致谢（模板） 14 正文 慢性低氧高二氧化碳对小鼠大脑线粒体功能的影响（临床医学资料） 文1：慢性低氧高二氧化碳对小鼠大脑线粒体功能的影响 Abstract: ? Objective: To explore the influence of chronic hypoxic hypercapnia on the mitochon-dria in the cerebrum of the mice.?Methods:?Thirty male C57BL/6 mice were randomly divided into two groups: the hypoxic hypercapnia 4-week experiment group (A group) and normal control group (B group). The experiment group was exposed to an atmosphere containing 9%～11% O2 and 5%～6% CO2 8 hou a day， 6 days a week for 4 weeks. The rest time A group lived in the room as B group did. The concentration of ATP，ADP and AMP was measured by HPLC. The changes of the ultramicrostructures in the mitochondria of the cerebrum were observed under the tramission electron membrane potential of the mitochondria was observed by the confocal and the inteity was measured by spectrophotofluorometry. The activity of the COXⅠor COXⅢ in the mitochondria and the SOD or GSH in the tissue of the cerebrum were measured by spectrophotofluorometry， respectively. Results:?Compared with the normal control group， the concentration of the ATP and AMP of mice was lower obviously (P). the ultramicrostructures were abnormal and the deity of the membrane potential decreased of the mitochondria，the activity of the COXⅠor COXⅢin the mitochondria of the cerebrum and the SOD or GSH in the cerebrum tiusse was also decreased significantly respectively (P). Conclusion:?Under the condition of exposue to chronic hypoxic hypercapnia，the dysfunction of the mitochondria in the mice’s cerebrum may occur. Its mechanism may be related to the free radical. Key words: ? mitochondria;hypoxia;hypercapnia;free radical;mice 慢性阻塞性肺病(chronic obstructive