参考成果分子molecular biology course-2b.pptxVIP

课件：白冠华 　;Consider the following complementation analysis.??Five mutations in a biosynthetic pathway (producing auxotrophs in a haploid state) were placed pairwise in a cell intrans?(diploid analysis).??The diploid cells were then assayed for reconstitution of the biosynthetic pathway; complementing mutations were able to grow in the absence of the end product of the pathway (i.e. they now had a functional biosynthetic pathway).??A + indicates a complementing pair of mutations; a - means that the two mutations did not complement. ? ????????????????????????????????????????????????????????????????????????????Mutation number ????????????????????????????????????????????????????????????????????????1??????????2??????????3??????????4??????????5 ? ????????????????????????????????????????????????1??????????????????????-???????????+?????????-??????????+?????????- ????????????????????????????????????????????????2??????????????????????????????????-??????????+?????????+?????????+ ????????????????????????????????????????????????3??????????????????????????????????????????????-??????????+?????????- ????????????????????????????????????????????????4??????????????????????????????????????????????????????????-??????????+ ????????????????????????????????????????????????5??????????????????????????????????????????????????????????????????????- ? ????????????a)????????Which mutations are in the same complementation group (representing mutant alleles of the same gene)? ????????????b)???????What is the minimal number of enzymatic steps in the biosynthetic pathway?;Genetic Control in Prokaryotes;Gene in Prokaryote;One promoter: several genes;Eukaryotic genes are often interrupted;Gene in Eukaryote ;Exons are the sequences represented in the mature RNA. By definition, a gene starts and ends with exons that correspond to the 5 and 3 ends of the RNA. Intron is a segment of DNA that is transcribed, but removed from within the transcript by splicing together the sequences (exons) on either side of