关于电针对实验性痴呆大鼠脑组织一氧化氮含量及神经性一氧化氮合酶表达的影响.docVIP

关于电针对实验性痴呆大鼠脑组织一氧化氮含量及神经性一氧化氮合酶表达的影响 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：关于电针对实验性痴呆大鼠脑组织一氧化氮含量及神经性一氧化氮合酶表达的影响 1 1 实验材料 3 2 实验方法 3 3 结果 6 4 讨论 7 文2：养心调肝益肾煎剂对去势雄鼠性活动及阴茎一氧化氮含量的影响 8 1 材料与方法 10 2 结果 11 3 讨论 12 参考文摘引言： 14 原创性声明（模板） 16 文章致谢（模板） 16 正文 关于电针对实验性痴呆大鼠脑组织一氧化氮含量及神经性一氧化氮合酶表达的影响 文1：关于电针对实验性痴呆大鼠脑组织一氧化氮含量及神经性一氧化氮合酶表达的影响 Abstract：Objective To explore the effect of electroacupuncture on the level of nitric oxide (NO) and neuronal nitric oxide synthase (nNOS) expression in cerebral tissues of demention rats. Methods Vascular dementia (VD) rat model of reperfusion following cerebral global ischemia was established by using a modified Pulsinelli-4-vessel occlusion method. All the rats were randomly pided into 4 groups：sham-operated control group， model group， electroacupuncture group， Piracetan group. Histological survey was carried out to detect the contents of NO in cerebral tissues after treatment. nNos expression in CA1 areas of hippocampus and other cerebral areas was detected and calculated by Leica-bx60 Image Analyzer. Y-type maze was used to measure learning and memory scores of the rats. Results The scores of Y-type maze of the model group were apparently less than sham-operated group (). Conclusio Electroacupuncture could apparently improve the ability of learning and memory of demention rats and the mechanism may be that electroacupuncture could improve antioxidative ability and inhibit active oxygen injury to relieve the cerebral tissues and the reaction of free radicals， and acupuncture could inhibit the over-increased nNOS after cerebral ischemia to protect the hippocampus neuro from decreasing。 Key words：electroacupuncture；demention；NO；neuronal nitric oxide synthase；animal model 现有研究表明，脑缺血损伤后学习记忆障碍可能多与中枢胆碱能系统、海马区缺血损伤、氧自由基、脂质过氧化物、单胺类递质、脑血流等改变有关[1]。笔者临床观察实验表明，电针对于治疗小儿智力低下(Mental Retardation，MR)有显著疗效。为了进一部探讨电针治疗MR的作用机理，本实验选取建立四血管阻断全脑缺血损伤后造成痴呆大鼠模型，采用五神针(百会穴前后左右旁开寸)、三智针(神庭穴加双侧本神穴)进行针刺，并用Y电迷宫观测大鼠学习记忆，选取检测脑组织一氧化氮(NO)含量变