高脂喂养联合免疫损伤建立兔动脉粥样硬化模型及评价.docVIP

高脂喂养联合免疫损伤建立兔动脉粥样硬化模型及评价 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：高脂喂养联合免疫损伤建立兔动脉粥样硬化模型及评价 1 1 对象与方法 3 2 结果 5 三组兔血脂水平比较 5 3 讨论 5 文2：光学干涉断层成像对球囊损伤联合高脂喂养兔腹主动脉粥样硬化模型 9 1 材料与方法 11 2 结果 13 3 讨论 14 参考文摘引言： 15 原创性声明（模板） 17 文章致谢（模板） 17 正文 高脂喂养联合免疫损伤建立兔动脉粥样硬化模型及评价 文1：高脂喂养联合免疫损伤建立兔动脉粥样硬化模型及评价 [Abstract] Objective To establish the experimental rabbit atherosclerosis plaque formation model by high fat feeding and immune injury， in order to improve the rabbit experimental atherosclerosis model. Methods 45 male New Zealand white rabbits were randomly divided into three groups. Normal control group （15 rabbits） was given ordinary feed； the high fat diet model group （15 rabbits） was given high fat forage （% ordinary feed +% egg yolk powder +6% cholesterol +4% lard）； the high-fat diet combined immune injury group （15 rabbits） was fed the same as high fat feeding model group， while rabbits were given intramuscular injection of fetal bovine serum protein （250 mg/kg） per week. Rabbit serum was collected for lipid Ⅰ indexes detection and femoral artery was probed with ultrasound for atherosclerotic aortic plaque detection at 12 weeks of feeding， then the rabbit aorta was taken out for pathological analysis by HE staining. Results Compared to normal control group， high fat diet model group and high fat diet combined immune injury model group increased significantly in serum biochemical indicato of total cholesterol， triglyceride， high deity lipoprotein cholesterol， and low deity lipoprotein cholesterol （P ）. The high fat diet combined immune injury model group showed larger pathological morphology compared with the high fat diet model group and normal control group， meanwhile femoral artery ultrasound showed more significant difference of lesio compared with the high fat diet model group and the normal control group. HE staining showed that， the high fat diet combined immune injury model group had more lipid foam cells， lymphocytes and fiber patches f