核磁共振氢谱对6群肺炎链球菌荚膜多糖的结构分析.docVIP

核磁共振氢谱对6群肺炎链球菌荚膜多糖的结构分析 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：核磁共振氢谱对6群肺炎链球菌荚膜多糖的结构分析 1 1 材料与方法 3 6群肺炎链球菌由中国医学细菌保藏管理中心保藏提供。 3 6群肺炎链球菌的血清型鉴定 4 2 结果 5 3 讨论 6 文2：肺炎链球菌转化模型的建立 6 0 引言 7 1 材料和方法 8 2 结果 10 3 讨论 11 参考文摘引言： 12 原创性声明（模板） 14 文章致谢（模板） 14 正文 核磁共振氢谱对6群肺炎链球菌荚膜多糖的结构分析 文1：核磁共振氢谱对6群肺炎链球菌荚膜多糖的结构分析 [Abstract] Objective To analyze the structures of the capsular polysaccharides from streptococcus pneumoniae serogroup 6. Methods Serotypes of streptococcus pneumonia were determinated as 6A， 6B， 6C and 6D using the molecular biological technologies， then the capsular polysaccharides were extracted and the structures were analyzed by 1H nuclear magnetic resonance （NMR）. The chemical shifts of anomeric proton regio and ring proton regio of 1H NMR spectrums were analyzed and compared among different serotypes of capsular polysaccharides. Results The chemical shifts of all characteristic proto peaks of the pneumococcal capsular polysaccharides serotype 6A were ？啄， ？啄 and ？啄 separately； serotype 6C were ？啄， ？啄， and ？啄； serotype 6B were ？啄， ？啄 and ？啄； serotype 6D were ？啄， ？啄， and ？啄. The chemical shifts of ring proton regio （？啄） of 4 serotypes were different. Conclusion The 1H NMR spectrums of pneumococcal capsular polysaccharides serotype 6A， 6B， 6C and 6D were determinated， and the chemical shifts of anomeric proton regio and ring proton regio were different in each serotype. It provides data for the quality control of different capsular polysaccharide。 [Key words] Pneumococcal capsular polysaccharide； 1H NMR； Anomeric proton； Ring proton； Chemical shift 肺炎链球菌（Streptococcus pneumoniae）是严重威胁人类健康的常见病原菌之一，能够引起肺炎、急性呼吸道感染、中耳炎、脑膜炎和菌血症等侵袭性和非侵袭性疾病。根据世界卫生组织（World Health Organization，WHO）报道，在发展中国家每年约有160万例死于肺炎链球菌感染，其中100万例是5岁的儿童[1] 肺炎链球菌荚膜多糖既是毒力因子又是保护性抗原，已成功用来制备肺炎链球菌荚膜多糖疫苗及多糖蛋白结合疫苗。6A和6B型肺炎链球菌作为导致侵袭性链球菌感染的常见血清型，23价肺炎球菌多糖疫苗和7价肺炎球菌多糖疫苗中均包含6B型荚膜多糖，10价和13价肺炎球菌结合疫苗中均包含6A和6B型荚膜多糖。随着分子生物学技术的进展，对荚膜多糖合成相关基因（cps loci）的深入研究，发现6群肺炎链球菌可分为6A、6B、6C、6D、6E、6F、