多聚左旋赖氨酸对大脑皮层星形胶质细胞体外培养的影响（医学资料）.docVIP

多聚左旋赖氨酸对大脑皮层星形胶质细胞体外培养的影响（医学资料） 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：多聚左旋赖氨酸对大脑皮层星形胶质细胞体外培养的影响 1 2 结果 5 3 讨论 6 文2：顺铂对正常星形胶质细胞的影响 8 1材料与方法 8 参考文摘引言： 10 原创性声明（模板） 11 文章致谢（模板） 12 正文 多聚左旋赖氨酸对大脑皮层星形胶质细胞体外培养的影响（医学资料） 文1：多聚左旋赖氨酸对大脑皮层星形胶质细胞体外培养的影响 [Abstract] Objective To investigate the effects of different concentratio of poly-L-lysine （PLL） on the cultures of cerebra lcorticalastrocytes （AS） in vitro. Methods Astrocytes were dissociated from cerebra lcortices with the methods of trypsinization and differential adherence. They were divided into four groups and respectively planted in culture flasks or plates which coated PLL of different concentratio： control group （non-PLL）， 25 μg/mL PLL envelop group， 50 μg/mL PLL envelop group， 100 μg/mL PLL envelop group. Adherent rates were detected by counted number and cell growth curves were drawn with CCK-8 colorimeter. To remove other cell types， the cells were shaken and passaged when they were confluent in a monolay on the bottom. Purities were assessed by immune of luorescence analysis of glial fibrillate acidic protein （GFAP） and cell viabilities were measured by CCK-8 colorimeter in the third generation astrocytes. Results Adherent rates were increased in a dose-dependent manner. Growth curves showed that the growth speed of control group was significantly slower than three PLL envelop groups. The purities of all groups were over 95% except 100 μg/mL PLL envelop group [（±）%]. The cell activities of control group on the fourth day and the sixth day both were lower than three PLL envelop groups， the differences were statistially signifiant （P ）. Conclusion PLL-coated technique can effectively promote adhesion and growth of AS， and the best coated concentratio are 25-50 μg/mL。 [Key words] Astrocyte； In vitro； Poly-L-lysine； Immunofluorescence； Glial fibrillary acidic protein 随着近年对大脑皮层星形胶质细胞（AS）的研究发现，AS以积极主动的方式参与调控突触传递，在突触重塑及学习记忆过程中起着重要的作用[1-2]。电生理技术的发展，也让人们了解到，AS存在着许多离子通道[3]，与神经元之间进行着积极的双向信息交流[4]。因此