神经干细胞移植对大鼠视神经损伤后节细胞的保护作用（医疗卫生论文资料）.docVIP

神经干细胞移植对大鼠视神经损伤后节细胞的保护作用（医疗卫生论文资料） 文档信息 ： 文档作为关于“医学心理学”中“眼科学”的参考范文，为解决如何写好实用应用文、正确编写文案格式、内容素材摘取等相关工作提供支持。正文9359字，doc格式，可编辑。质优实惠，欢迎下载！ 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：神经干细胞移植对大鼠视神经损伤后节细胞的保护作用 2 1 材料与方法 3 2 结 果 6 3 讨 论 6 文2：纳洛酮对少突胶质细胞的保护作用 8 1 材料和方法 8 2 结果 10 3 讨论 11 参考文摘引言： 12 原创性声明（模板） 13 文章致谢（模板） 14 正文 神经干细胞移植对大鼠视神经损伤后节细胞的保护作用（医疗卫生论文资料） 文1：神经干细胞移植对大鼠视神经损伤后节细胞的保护作用 Protective effect of neural stem cell traplantation on retinal ganglion cells of rats with optic nerve injury LIU Haoran1， YANG Changhong2， GAO Junwei1， et al 1. Department of Neurosurgery， Xiangya Hospital of Central South Univeity， Changsha 410008， China; 2. Department of Neurosurgery， Hospital for Guangdong Frontier General Team， Chinese Peoples Armed Police Forces， Shenzhen 518029， China Abstract: Objective To investigate the protective effect of neural stem cell (C) traplantation on retinal ganglion cells (RGCs) of Sprague-Dawley rats with optic nerve injury. Methods Forty-eight adult Sprague-Dawley rats were randomly divided into group N (C) and group C (control). Optic nerve crush injury was induced in the right eyes by a calibrated method， and the left eyes served as the normal control. Cs were taken from embryonic SD rat hippocampus and cultured in vitro and grafted in vivo. Cs were injected into the vitreous body of right eyes of rats in group N. Rats in group C were injected with isovolumetric PBS. Three days before sacrifice， 3% fast blue was injected into the superior colliculi bilaterally. According to the time interval between the optic nerve crush and sacrifice， both group N and C were divided respectively into four subgroups (day 7， day 14， day 21 and day 28) with 6 rats in each one. The eyes were enucleated after the rat was sacrificed， and flat mounts of the retina from both eyes were observed under a fluorescence microscope. The labeled RGCs were counted by a computerized image analyzer. The label rate of RGCs was calculated for statistical ana