ROCK2调控骨肉瘤细胞的侵袭及迁移.pdfVIP

ROCK2调控骨肉瘤细胞的侵袭及迁移 Abstract: Objective To analyze the expression of ROCK2 in osteosarcoma tissues and explore its effect on the invasion and migration of osteosarcoma cells and related mechanism. Methods Real-time fluorescent quantitative PCR, Western blot and immunohistochemical staining were used to detect the expression of ROCK2 in osteosarcoma tissues. The migration and invasion of osteosarcoma cells were analyzed by wound- healing and Transwell assays after the knockdown of ROCK2 expression. The effect of reducing ROCK2 expression on metastasis in vivo was tested by tail vein metastasis experiment. We detected the expression of EMT-related proteins in osteosarcoma cells, and added the EMT inducer TGF-β to osteosarcoma cells with down-regulated ROCK2 expression to detect the changes of cell invasion and migration. Results The expression of ROCK2 in osteosarcoma tissues was significantly higher than that in corresponding adjacent tissues (P0.05). After down-regulating the expression of ROCK2 in osteosarcoma cells, the invasion and migration abilities in vivo and in vitro were significantly inhibited (P0.05). The expression of EMT-related protein E-cadherin decreased, while N-cadherin and Vimentin proteins increased significantly. Inducing EMT could attenuate the inhibitory effect of down-regulating ROCK2 on the invasion and migration of osteosarcoma cells. Conclusion The expression of ROCK2 is increased in osteosarcoma tissues, which promotes the invasion and migration of osteosarcoma cells by inducing EMT. ROCK2 may be a potential biomarker for targeted therapy of osteosarcoma. Key words: ROCK2; EMT; Osteosarcoma; Invasion; Migration ： 探讨骨肉瘤组织中ROCK2 的表达对骨肉瘤细胞侵袭及迁移的影响及其作用机制。 摘 要 目的 方法 采用实时荧光定量PCR 、Western blot 及免疫组织化学染色法检测骨肉瘤组织中ROCK2 的 表达；沉默骨肉瘤细胞中ROCK2 的表达后，采用划痕愈合及Transwell 实验分析骨肉瘤细胞的侵袭 及迁移能力，并通过尾静脉转移实验检测降低ROCK2表达对体内转移的影响。检测骨肉瘤细胞中 EMT ROCK2 EMT