四磨汤与功能性消化不良模型大鼠胃RhoA及ROCK的关系研究.pdfVIP

四磨汤与功能性消化不良模型大鼠胃RhoA 及 ROCK 的关系研究 摘要：目的 研究四磨汤与功能性消化不良模型大鼠胃 RhoA 及 ROCK 的关系，探讨其治疗机制。方法 通 过碘乙酰胺灌胃、孤养及平台站立剥夺睡眠制备功能性消化不良大鼠模型。观察大鼠的一般状态，通过旷场试验 和糖水试验鉴定大鼠模型。采用胃平滑肌肌条收缩实验检测抑制剂 Y27632 和四磨汤对胃平滑肌肌条作用。采用 荧光定量PCR 法检测大鼠胃组织RhoA 及 ROCK 表达情况。结果 构建完成功能性消化不良大鼠模型；抑制剂 Y27632 干预了四磨汤对胃平滑肌肌条的增强作用；荧光定量PCR 结果显示模型组RhoA 及 ROCK 基因 mRNA 的 表达较正常组表达降低且有统计学意义（ 0.05 0.01 RhoA ROCK P ＜ ；P ＜ ）；四磨汤干预后，四磨汤组 及 基因 mRNA 的表达较模型组表达升高且有统计学意义（ 0.01 0.05 P ＜ ；P ＜ ）。结论 成功构建功能性消化不良大 RhoA ROCK 鼠模型。 及 的表达可能与四磨汤对功能性消化不良作用有相关性。 关键词：功能性消化不良；四磨汤；RhoA；ROCK Study on the relationship between Simo Decoction and the expression of gastric RhoA and ROCK in rats with functional dyspepsia Abstract: Objective To study the relationship between Simo Decoction and the expression of gastric RhoA and ROCK in rats with functional dyspepsia, and to explore its therapeutic mechanism. Methods A rat model of functional dyspepsia was established by intragastric administration of iodoacetamide, solitary rearing and sleep deprivation by platform standing. The general state of the rats was observed, and the model was identifi ed by the open fi eld test and the sugar water test. The eff ect of the inhibitor Y27632 and Simo Decoction on gastric smooth muscle strips was detected by gastric smooth muscle strip contraction tests. The expression of RhoA and ROCK in rat gastric tissues was detected by fl uorescence quantitative PCR. Results The functional dyspepsia rat model was constructed. The inhibitor Y27632 interfered with the enhancement eff ect of Simo Decoction on gastric smooth muscle strips. The results of fl uorescence quantitative PCR showed that the expression of RhoA and ROCK gene mRNA in the model