罗格列酮双模免疫传感器构建.docxVIP

PAGE 2 摘 要 　　本文构建了一种新型罗格列酮双模免疫传感器。采用双电极智能传感装置，实现了便携式传感。此外，制备了具有优良过氧化物酶活性的八面体Cu2O（o-Cu2O）与金纳米粒子的复合材料（o-Cu2O@Au）作为模拟酶，避免了天然酶成本高，稳定性差的缺点。在H2O2存在下，4-氯-1-萘酚能被催化，在生物电极上形成氧化沉淀，显著改变电极电阻，引起电化学信号响应。在比色通道中，普鲁士蓝（PB）薄膜在还原为普鲁士白（PW）时，会经历由蓝变白的过程。颜色变化与修饰在生物电极上的生物分子的数量有关，因此可以建立色度值（B/（R+G+B））与罗格列酮浓度之间的定量关系，线性范围为5×10-10至5×10-7 gL-1，检测限（LOD）为1.27×10-10 gL-1（S/N=3）。在计时通道中，电位达到一定值所需的时间(Δt）用智能程序记录，并建立了Δt和罗格列酮浓度的回归方程。线性范围为5×10-10 gL-1至5×10-7 gL-1，检出限为0.82×10-10 gL-1（S/N=3）。双模传感方法具有良好的稳定性和可靠性，与小型便携式设备一起在实际应用中显示出良好的应用前景。 关键词：罗格列酮免疫传感器电致变色双信号检测便携式仪器多信号检测 　　Application of Dual-signal Biosensor Based on Novel Mimetic Enzyme in the Detection of Rosiglitazone 　　Abstract: In this work, a novel dual-mode immunosensor of rosiglitazone was constructed. Using a smart device with a two-electrode system, a portable sensing was realized. Besides, a composite of octahedral Cu2O (o-Cu2O) and Au nanoparticles (o-Cu2O@Au) with excellent peroxidase activity was used as the mimetic enzyme, which prevented the usage of natural enzyme with high cost and poor stability. In the presence of H2O2, 4-chloro-1-naphthol could be catalyzed and an oxidative precipitation could be formed on bioelectrode, which significantly changed the electric resistance and caused the electrochemical signal respond. In the colorimetric channel, prussian blue (PB) thin film could go through a color change from blue to white as it was reduced to prussian white (PW). The color variation was related to the amounts of biomolecules modified on the bioelectrode, therefore a quantitative relationship between the chroma value (B/(R+G+B)) and rosiglitazone concentration could be established, in which a linear range of 5×10-10 gL-1-5×10-7 gL-1, and a limit of detection (LOD) of 1.27×10-10 gL-1 (S/N=3) was achieved. While in the timing channel, the time taken for the potential to reach a certain value (Δt) was recorded with a smart program, and a regression curve was built between Δt and rosiglitazone concentration. The linear range was from 5×