二零二三年 优质公开课Gaschromatography.pptxVIP

Pharmaceutical analysis Gas chromatography Gas chromatography Author: Xu Yingqian Contents Section I Overview Section II Basic theory of gas chromatography Section III Stationary and mobile phases of gas chromatography Section IV Qualitative and quantitative analysis Key and difficult points Master Familiar Qualitative and quantitative analysis methods of gas chromatography. Concept of gas chromatography, stationary phase and mobile phase. UnderstoodBasic theory of gas chromatography. Section I Overview Gas chromatography is a chromatographic separation and analysis method with gas as a mobile phase. Gas chromatography is widely used in petrochemical, medical and health, environmental science, organic synthesis, bioengineering and other fields. In pharmaceutical analysis, gas chromatography has become an important separation and analysis method in content determination, impurity detection, Chinese herbal medicine composition analysis, drug purification, preparation, etc. 1. Gas chromatograph Gas chromatograph generally consists of five parts, as shown in the following figure: 1. Gas chromatograph (1) Carrier gas system, including a gas source, a gas purifier, a gas flow velocity control and a measuring device. (2) Sample injection system, including a sample injector, a gasification chamber and a temperature control device. (3) Separation system, including chromatographic column and chromatographic column box. (4) Detection system, including detector and temperature control device. (5) Recording system, including an amplifier, a recorder or a data processing device. 2. Chromatographic outflow curve and its common terms The response signal produced by the components flowing from the chromatographic column as they pass through the detector is called chromatographic outflow curve, and is also called chromatogram. Generally, the chromatogram takes the time of components or the volume of mobile phase as the abscissa, and the response signal of the detector as t