东南大学电子学院《现代光学基础》补充内容3显微镜.pptVIP

OUTLINE7.1 Optical Microscopy7.2 Confocal Microscopy7.3 Applications of Confocal Microscopy in Biomedicine当前第1页\共有73页\编于星期二\22点 7.1 Optical MicroscopyThree goals: produce a magnified image of the specimen, separate the details in the image,render the details visible to the human eye or camera. Simple single lens devices that are often hand-held, such as a magnifying glass. Multiple-lens designs with objectives and condensers (compound)当前第2页\共有73页\编于星期二\22点 Microscopy: HistorySimpleCompound当前第3页\共有73页\编于星期二\22点 Microscopy: History当前第4页\共有73页\编于星期二\22点 Microscopy: History当前第5页\共有73页\编于星期二\22点 Microscopy: ImportanceBiomedical sciences: overall morphological features of specimens; quantitative tooladvances in fluorochrome stains and monoclonal antibody techniques: explosive growth in the use of fluorescence microscopy in both biomedical analysis and cell biology.optical microscope most important biomedical opticExplosive growth in physical and materials sciences; semiconductor industry, observe surface features of high-tech materials and integrated circuits Forensic scientists: hairs, fibers, clothing, blood stains, bullets, and other items associated with crimes当前第6页\共有73页\编于星期二\22点 Simple MagnificationSo 2fSo 2fSo = 2ff So 2f当前第7页\共有73页\编于星期二\22点 Image formation on Retinado~25cm当前第8页\共有73页\编于星期二\22点 Combination of Lens and EyeSimple microscope: bi-convex lens, image perceived by eye as if it were at a distance of 10 inches or 25 centimeters (near point)Image appears on same side of lens as object, cannot be projected onto a screen: virtual image (upright, not inverted). Light reflected from the rose enters the lens in straight lines, refracted and focused by the lens to produce a virtual image on the retina.Image of the rose magnified: perceive actual size of object to be at infinity; eyes trace light rays back in straight lines to virtual image 当前第9页\共有73页\编于星期二\22点 Combination of Lens and EyeSofSilLMP = doD at l = f;Decrease l or L, increase MP:MP = doD + 1 at l