动物产品中腹泻性贝类毒素检测方法研究.pdfVIP

糾畜希欢浚矗 I试验研究I 2022年第5期(总第231期) doi:10.3969/j.issn.1006-4907.2022.05.015 动物产品中腹泻性贝类毒素检测方法研究 陈盼1 ,胡忆文2 ,侯义宏2,禹思宇3陆静谭建锡2笃唐连飞2探 (1.长沙县食品药品安全检测中心，湖南长沙410010; 2 .长沙海关技术中心，湖南长沙410004) 摘 要：为探讨小分子核酸适体快速检测动物产品中腹泻性贝类毒素的可行性，研究基于冈田酸(Okadaic acid, OA)核酸适体， 构建了特异性适体辅助识别体系平台 。结果显示 ，当体系中靶标OA被释放后，导致可结合标记酶催化产生不同程度 的颜色反应，可以用肉眼进行初步判断；通过波长扫描显示在450 nm波长呈现最大吸收峰，测定其吸光度值,可反映 出溶液中的靶标浓度 。该体系平台方法具有操作步骤简便、对检测硬件要求低等优点，可用于基层养殖现场快速初筛 检测中的使用o 关键词：腹泻性贝类毒素;核酸适体;吸光度 中图分类号汀S254.7 文献标识码:B 文章编号：1006-4907( 2022)05-0039-03 Study on the Detection Method of Diarrhea Shellfish Toxin in Animal Products CHEN Pan1, HU Yi-Wen2, HOU Yi-Hong2, YU Si-Yu2, LU-Jing2, TAN Jian-Xi2*, TANG Lian-Fei2* (1. Changsha County Food and Drug Scfety Testing Center, Changsha 41 10, PRC; 2. Changsha Customs Technology Center, Changsha 41 4, PRC) Abstract: In order to explore the feasibility of small molecule aptamer for rapid detection of diarrhea shellfish toxins in animal products, this study constructed a specific aptamer assisted recognition system platform based on Okadaic acid (OA) aptamer. The results showed that when the target OA in the system was released, it led to the color reaction of different degrees in the reaction catalyzed by the labeled enzyme, which can be preliminarily judged by the naked eye; The wavelength scanning shows that there is a maximum absorption peak at 450 nm wavelength. The absorbance value can reflect the target concentration in the solution. This system platform method has the advantages of simple operation steps and low requirements for detection hardware, and can be used in the rapid preliminary screening detection of breeding sites. Key words: Diarrhea shellfish toxin; Aptamer; Absorbance 冈田酸(Okadaic acid, OA),又被称为大田软海 ligands by expon