药理学教学课件：药理学实验.pptVIP

* * Methods Divide 4 mice into 2 groups (A and B) randomly (each group has 2 mice), weight and mark them. Inject intraperitoneally normal saline (0.1 ml/10g) to the mice of group A, and 0.2% diazepam 10 mg/kg to group B, respectively. 20 minutes later, inject intraperitoneally 0.15% picrotoxin (7.5 mg/kg) to each animal, and record the initiation time of clonic convulsion and the number of mice demonstrating clonic convulsion in 20 minutes. Compare the difference in results between the control group and the tested group and judge whether the tested drug have the anticonvulsant activity. The standard appearance of picrotoxin-induced convulsion: clonic convulsion after 5-15 minutes or excitability jump, then forelimb flection, hindlimb rigidity. Notices Be careful with the dose and the performance of the intraperitoneal injection (location and depth) in order to avoid the damage of the abdominal organs. After the typical phenomenon is observed, kill the animal immediately to relieve the pain of the animal. Questions What kinds of epilepsy can be treated with diazepam? What is the underlying antiepileptic mechanism? Are there any other clinical applications of diazepam? * 苯巴比妥和苯妥英钠的 抗电惊厥作用 【有关知识】 苯巴比妥 抑制病灶神经元异常放电及其扩散，主要用于 癫痫大发作及其持续状态 苯妥英钠 阻止病灶异常放电向正常脑组织扩散，降低细胞膜 对钠离子、钙离子的通透性，抑制PTP形成。主要用于 1 大发作、精神运动性发作 首选 2 三叉神经痛、舌咽神经痛 3 心律失常（室性） * 【操作步骤】 设定条件：电压30V。刺激周期：单次， 刺激时间：0.3S 选择电惊厥阳性鼠35只，称重后分7组； 各组分别IP NS、苯巴比妥3组（0.1 % 、0.2 % 、0.4%）、苯妥英钠3组（ 0.1 % 、0.2 % 、0.4% ） 剂量均为 0.1ml/10g 30分钟后，原条件再给予电刺激， 记录强直性屈曲、伸展等的持续时间 * 【注意事项】 小鼠电惊厥指标：前肢屈曲、后腿伸直； 鳄鱼夹需沾生理盐水，以利导电； 3 用药前后小鼠电刺激条件要一致。 Experiment 7 Effects of phenobarbital and phenytoin on electrofit in mice Purpose Comparatively study the anticonvulsive action of phenobarbital and phenytoin, and determine the dose-res