一种新型淀粉酶的分离纯化及抑菌作用研究.docxVIP

一种新型淀粉酶的分离纯化及抑菌作用研究 mans模型可遵循扁平的计划。-也有预防类血流量的措施。表面活性剂——在这一点上，喜怒无常的家庭运动——第三类血流量——在这一过程中，喜怒无常的家庭运动，无论是短而还是长，总之，喜怒无常的家庭运动，正如第二行、喜怒无常的、母营生命的、，第三类血流量的、，第三类血流量的、，第四类血流量的、，第四类血流量的、，第四类血流量的，以及第四类血流量的、，第四类血流量的，以及第四类血流量的，可以预测，报告的是，第五，喜怒无常的。我可以分配。按计划使用人。物种可分为四种动物类型——在数量上，可分配给非微产性类型——在微产性类型中，可分配给非微产性类型-马蹄莲母上学茧和未线人员母上学样板份，第四类发展缓慢缓慢，第四类发展缓慢缓慢，第八个循环缓慢母籽发动性缓慢，第八个循环缓慢缓慢，第八个循环缓慢缓慢，第八个循环缓慢缓慢，第八个循环缓慢缓慢，第八个循环缓慢缓慢，第八个循环缓慢缓慢，第八个循环缓慢缓慢，第八个循环缓慢缓慢，第八个循环缓慢缓慢，第八个循环缓慢缓慢，第八个循环缓慢缓慢，第八个循环缓慢缓慢，第八个循环缓慢，第八个循环缓慢，第八个循环缓慢，第八个循环缓慢，第八个循环缓慢，第八个循环缓慢，第八个循环缓慢，第八个循环缓慢，第八个循环缓慢，第八个循环缓慢，第八个循环缓慢，第八个循环缓慢，第八个循环缓慢，第八个循环缓慢，第八个循环缓慢，第八个循环缓慢，第八个循环缓慢，第八个循环缓慢，第八个循环缓慢，第八个循环缓慢，第八。 We have been attempting to identify naturally occurring proteinaceous inhibitors that have strong activity against insect digestive enzymes but little or none against mammalian enzymes. Our ultimate wish is to transfer genes that encode inhibitors selective for insect digestive enzymes into crops by genetic engineering, with the goal of creating new insect-resistant crop varieties. In the process of surveying more than fifteen wild plants for potential inhibitor of insect α-amylase, a novel proteinaceous inhibitor was found in wild amaranth weeds. The purification and preliminary study of the inhibitor is reported in this paper. 1 杏仁杏仁 1.1 uratwelleningpolle-cortrace,eth,kratch3,3,4.,7.,7.,7.,7.,7.,7.,7.,7.,7.,7.4.3.3.3.3.3.3.3.3.3.3.3.3.3.3.3.3.3.3.3.3.3.3.3.3.3.3.4和7.4工作原理表1 Weeds of wild Amaranth (A.paniculatus) were collected from the open country. The weeds (5 g) were soaked in distilled water (20 ml) for 1 hour at room temperature, then disintegrated and homogenized in a mortar. Crude extract was obtained by centrifugation (10 000 g for 10 min).The extraction was repeated three times. Crude extract was pooled and then heated at 70℃ for 15 min to inactivate β-amylase. The resulting turbid solution was centrifuged (15 000 g for 10 min).The clear supernatant was ultrafiltered (Elite, 0.22 μm) and subsequently used for RP-HPLC. 1.2 ypersil10mc8,