韭菜中吡虫啉和啶虫脒残留的微波处理逆固相分散法净化及液相色谱检测.docx

韭菜中毗虫琳和啶虫脒残留的微波处理逆固相分散法净化及液

相色谱检测

作者:作者：陈黎王金芳杜鹏唐小革赵坤霞潘灿平作者单位：(中国农业大学理学院，北京100094)来源：医学期刊/基础医学与生物医学工程

【摘要】建立了韭菜中两种烟碱类农药毗虫琳和啶虫豚残留的快速检测方法。韭菜样本用微波炉加热处理，使酶钝化消除含硫基质干扰，然后用乙腈提取、逆固相分散净化，用反相高效液相色谱二极管阵列检测器检测。在0.05?2.0mg/kg添加水平范围内，毗虫琳的平均添加回收率在95.2%?105.3%之间；相对标准偏差在0.8%?7.8%之间；啶虫豚的平均添加回收率在97.4%?108.8%之间；相对标准偏差在1.3%?8.3%之间。本方法对此虫琳的检出限(LOD)为0.0078mg/kg，定量检出限(LOQ)为0.026mg/kg；对啶虫月米的检出限为0.0075mg/kg，定量检出限为0.025mg/kgo

【关键词】 韭菜，烟碱类农药，微波处理，逆固相分散，高效液相色谱

DeterminationofImidaclopridandAcetamipridinLeekbyReversedPhaseHighPerformanceLiquidChromatographywithMicrowaveTreatmentandConvenientReverseSolidPhaseDispersionCleanup

CHENLi,WANGJinFang,DUPeng,TANGXiaoGe,ZHAOKunXia,PANCanPing

(CollegeofSciences,ChinaAgriculturalUniversity,Beijing100094)

(QualitySupervision,InspectionandAnalysisCenterofAgroproducts,MinistryofAgriculture,Beijing100083)

AbstractArapidmethodhasbeendevelopedforthedeterminationoftwoneonicotinoidinsecticideresiduesincludingimidaclopridandacetamipridinleek.Thesamplesofleekweretreatedwithmicrowavetomaketheenzymeinactiveandtoeliminatethematrixinterferences.Thenthepretreatedsampleswereextractedwithacetonitrile,furthercleanedupbyreversesolidphasedispersion,andanalyzedbyreversedphaseHPLCwithdiodearraydetector(DAD).Averagerecoveriesofimidaclopridandacetamipridwerefoundintherangeof95.2%—105.3%and97.4%—108.8%atthreespikinglevelsfrom0.05mg/kgto2.0mg/kgwithrelativestandarddeviationsof0.8%—7.8%and1.3%—8.3%,respectively.Limitofdetectionsofimidaclopridandacetamipridwere0.0078mg/kgand0.0075mg/kg,whilelimitofquantificationswere0.026mg/kgand0.025mg/kg,respectively.

KeywordsLeek,neonicotinoidinsecticide,microwavetreatment,reversesolidphasedispersion,reversedphasehighperformanceliquidchromatography

本文系国家^一五科技攻关项目（No.2006BAK02A04）和北京市科技新星计划（No.2005A59）资助项目

*Email:canpingp@cau.edu.cn

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