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EnzymeandMicrobialTechnology30(2002)319–326/locate/enzmictec
Purificationandcharacterizationofnewendo-1,4--D-glucanasesfrom
Rhizopusoryzae
a,aaa
KoichiroMurashima*,TomokoNishimura,YukoNakamura,JinichiroKoga,
TastukiMoriyabbca
,NaomiSumida,TakashiYaguchi,ToshiakiKono
aBioScienceLaboratories,MeijiSeikaKaisha,Ltd.,Saitama,Japan
bPharmaceuticalTechnologyLaboratories,MeijiSeikaKaisha,Ltd.,Kanagawa,Japan
cPharmaceuticalResearchCenter,MeijiSeikaKaisha,Ltd,Kanagawa,Japan
Received1May2001;receivedinrevisedform3September2001;accepted5October2001
Abstract
Newextracellularendoglucanases,designatedRCE1andRCE2,producedbyRhizopusoryzaeisolatedfromthesoil,werepurifiedto
apparenthomogeneityfromtheculturesupernatant.ThemolecularmassofRCE1andthatofRCE2werefoundtobe41kDaand61kDa,
respectively.TheN-terminalaminoacidsequencesofRCE1andRCE2showedhighhomologywiththoseofthefamilyIcellulose-binding
domains.InternalaminoacidsequencesofRCE1andRCE2showedhomologywiththatofthecatalyticdomainofEGVfromHumicola
insolensbelongingtofamily45endoglucanase.ThecellooligosaccharidehydrolysispatternsofRCE1andRCE2weresimilartothatof
EGVfromH.insolens.TheseresultsindicatethatRCE1andRCE2arefamily45endoglucanaseshavingacellulosebindin
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