质粒22010pbifc-vn173基因片段名称替代venus.pdfVIP

Plasmid22010:pBiFC-VN173

Gene/insertname:

VN173

Altname:

Venus(1-172)

Insertsize:

516

Species:

Aequoreavictoria

Fusionproteinortag:

FLAG

Terminal:

Nterminalonbackbone

Mutation:

Venusresidus1-172

Vectorbackbone:

pFlag-CMV

(SearchVectorDatabase)

Backbonemanufacturer:

Sigma

Vectortype:

MammalianExpression

Backbonesizew/oinsert(bp):

4700

Cloningsite5:

XbaI

Sitedestroyedduringcloning:

No

Cloningsite3:

BamHI

Sitedestroyedduringcloning:

No

5sequencingprimer:

CMV-FListofSequencingPrimers

3sequencingprimer:

hGH-PA-R

Bacterialresistance(s)

Ampicillin

Growthstrain(s)

DH5alpha

Growthtemperature(℃):

37

Highorlowcopy:

HighCopy

Sequence:Viewsequences(2)

PrincipalInvestigator:

Chang-DengHu

TermsandLicenses:

MTA

Plasmid22012:pBiFC-bJunVN173

Gene/insertname:

bJunVN173

Altname:

c-Jun(257-334)-Venus(1-172)

Insertsize:

774

Species:

R.norvegicus(rat);AequoreaVictoria

Fusionproteinortag:

FLAG

Terminal:

Nterminalonbackbone

Mutation:

c-Jun(257-338)-Venus(1-172)

Vectorbackbone:

pFLAG-CMV

(SearchVectorDatabase)

Backbonemanufacturer:

Sigma

Vectortype:

MammalianExpression

Backbonesizew/oinsert(bp):

4646

Cloningsite5:

EcoRI

Sitedestroyedduringcloning:

No

Cloningsite3:

BamHI

Sitedestroyedduringcloning:

No

5sequencingprimer:

C