大蒜素溶剂提取工艺优化.docx

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大蒜素溶剂提取工艺优化

【摘要】

目的:使用正交设计实验摸索溶剂提取法提取大蒜中大蒜素的最佳提取工艺。方法:使用溶剂提取法，第一部，通过文献检索、预实验和单因素实验摸索大蒜素提取条件及影响因素水平；第二步，正交设计实验的方法，对酶解温度、料液比、提取时间及提取次数对大蒜素提取率的影响进行考察，得到不同测定方法下的最佳提取工艺；第三步，通过比较两种检测方法提取率等数值，得到最适合的检测方法，得到最适合的溶剂提取法提取工艺。结果：该提取方法的最佳检测方法为高效液相色谱法，最佳提取工艺为酶解时间为40℃，料液比为1:4，提取时间为120min，提取次数为2次。结论:采用该提取方法提取大蒜中的大蒜素，实验方法全面客观，提取率高，基本符合大生产的实际需要，通过重复性实验证明该工艺稳定，值得推广。【关键词】大蒜素；溶剂提取法；高效液相色谱法；气相色谱法；最佳工艺

StudyOnPreparationOfCompoundCinnamomumLongepaniculatumOilGel

Abstract：

Objective:Orthogonaldesignexperimentwasusedtoexplorethebestextractionprocessofalliciningarlicbysolventextractionmethod.Methods:Usingsolventextractionmethod,thefirstpartistoexploretheextractionconditionsandinfluencingfactorsofallicinthroughliteraturesearch,pre-experimentandsinglefactorexperiment;thesecondstepisthemethodoforthogonaldesignexperiment,thetemperatureofenzymatichydrolysis,theratioofmaterialtoliquid,andextractionTheinfluenceoftimeandextractiontimesontheextractionrateofallicinwasinvestigated,andthebestextractionprocessunderdifferentdeterminationmethodswasobtained;thethirdstep,bycomparingtheextractionratesofthetwodetectionmethodsandothervalues,themostsuitabledetectionmethodwasobtained,andthemostsuitableTheextractionprocessofsolventextractionmethod.Results:Thebestdetectionmethodofthisextractionmethodishighperformanceliquidchromatography,andthebestextractionprocessisthattheenzymatichydrolysistimeis40℃,thematerial-liquidratiois1:4,theextractiontimeis120min,andthenumberofextractionsis2times.Conclusions:Usingthisextractionmethodtoextractalliciningarlic,theexperimentalmethodiscomprehensiveandobjective,theextractionrateishigh,andbasicallymeetstheactualneedsoflarge-scaleproduction.Repeatedexperimentsprovethattheprocessisstableandworthyofpromotion.

Keywords:Allicin;Sol