医学论文：炒白术和炒枳实混合co2超临界萃取工艺研究及gc-ms分析.docVIP

【摘要】? 目的 研究炒白术和炒枳实超临界co2混合萃取最佳工艺及其萃取物的化学成分。方法 采用正交设计方法,以萃取压力、萃取温度、co2流量、萃取时间为因素,考察超临界co2萃取的最佳工艺条件并进行gc-ms分析。结果 超临界co2萃取最佳工艺为：萃取压力33 mpa,萃取温度40 ,co2流量27 l/h,萃取时间60 min。所得萃取物主要成分为γ-榄香烯。结论 利用超临界co2萃取技术萃取炒白术和炒枳实,可以缩短萃取时间,明显提高收率。 【关键词】? 炒白术；炒枳实；超临界co2萃取；正交设计；gc-ms分析 ??? abstract：objective to study the optimum parameters of the supercritical co2 fluid extraction of roasted rhizoma atractylodis macrocephalae and roasted fructus aurantii immaturus, and chemical constituents of extractive matters. methods the experiment was performed with orthogonal design. four factors were extractive pressure, temperature, extractive time and the flow rate of co2. gc-ms was applied for analyzing. results the optimum condition were obtained：the extractive pressure was 33 mpa, the temperature of extraction was 40 ℃, the extractive time was 60 minutes and the flow rate of co2 was 27 l/h. the main chemical constituents was gamma-elemene. conclusion the method applied to obtain the extractive matters from roasted rhizoma atractylodis macrocephalae and roasted fructus aurantii immaturus was quickly and efficiently with satisfactory results. it provides foundation for exploration. ??? key words：roasted rhizoma atractylodis macrocephalae；roasted fructus aurantii immaturus；sfe-co2；orthogonal design；gc-ms?　　白术,为菊科植物白术atractylodes macrocephala koidz.的干燥根茎,味苦、甘,性温,归脾、胃经,具有健脾益气、燥湿利水、止汗、安胎的功效,麸炒后缓和了“燥性”,更注重于健脾补气和胃1。枳实,为芸香科植物酸橙citrus aurantium l.及其栽培变种或甜橙citrus sinensis osbeck的干燥幼果,具有补脾、燥湿、和中的功效,麸炒后偏于理气健胃消食。药理实验证明,上述两种麸炒药材混合萃取物对于治疗脾胃虚弱、气滞食积具有显著的药理活性,为了工业大生产实际需要,我们对这两种药材按照处方比例进行了超临界co2萃取,摸索最佳萃取条件,并对其成分进行分离测定。　　1? 实验材料　　1.1? 药物和试剂 ??? 炒白术、炒枳实(购自安徽井泉集团中药饮片有限公司),经江苏省药品检验所中药室董小平教授鉴定为菊科植物白术(rhizoma atractylodis macrocephalae)和芸香科植物枳实(fructus aurantii immaturus)。co2为食品级(南通市气体厂)；所用试剂均为分析纯。　　1.2? 仪器 ??? ha121-50-01型超临界co2萃取仪(南通市华安超临界萃取有限公司)；hp-6890/5973msd型气-质联用仪(美国惠普公司),nist98谱库。agilent7683自动进样器。　　2? 方法与结果　　2.1? 超临界co2萃取炒白术和炒枳实混合萃取物　　? 试验方法? 　　将炒白术、炒枳实粉碎(过20目筛),按照处方比例称取一定量投入1 l萃取釜中,加热萃取釜和解析釜,使之达到预定的温度,送入一定流速的c