柚皮苷对脂多糖诱导的beagle犬急性肺损伤的保护作用.docVIP

  柚皮苷对脂多糖诱导的 beagle 犬急性肺损 伤的保护作用# 聂怡初，李沛波，邹威，罗钰龙，苏薇薇** 5 10 15 （中山大学，生命科学学院，广州，510275） 摘要：目的：研究柚皮苷对脂多糖诱导的 beagle 犬急性肺损伤的保护作用。方法：beagle 犬 18 只，随机分为安慰剂组、LPS+安慰剂组、LPS+柚皮苷组。LPS+柚皮苷组按 12.4 mg/kg 的剂量给予柚皮苷胶囊，安慰剂组与 LPS+安慰剂组给予空白胶囊壳。给药 1 h 后，LPS+安 慰剂组与 LPS+柚皮苷组气管滴注脂多糖 4 mg/kg 构建急性肺损伤模型。造模 8 h 后取血和 支气管肺泡灌洗液及肺部组织，检测肺湿干比、痰液中总蛋白、Cl-、K+和 Na+含量、肺组 织中性粒细胞髓过氧化物酶(myeloperoxidase，MPO)和诱导型一氧化氮合酶(inducible nitric oxide synthase，iNOS)的活性、支气管肺泡灌洗液和痰液中白介素-8(interleukin-8，IL-8)与肿 瘤坏死因子(tumor necrosis factor-α，TNF-α)的含量，并采用 HE 染色观察肺部组织病理变 化。结果：柚皮苷能显著降低肺组织的湿干比、iNOS 和 MPO 活性，减轻肺部白细胞及中 性粒细胞浸润、抑制 LPS 引起的 TNF-α 与 IL-8 分泌、减轻肺部组织炎性病理损伤与出血渗 出。结论：柚皮苷预处理对 LPS 所致的急性肺损伤具有保护作用。 关键词：急性肺损伤；柚皮苷；脂多糖；beagle 犬；抗炎作用 中图分类号：R285.5 20 Protective effects of naringin on lipopolysaccharide induced acute lung injury in beagle dogs Nie Yichu, Li Peibo, Zou wei, Luo yulong, Su weiwei (School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275) 25 30 35 40 Abstract: Objective: To observe the potential protective effects of naringin on the lung tissue in lipopolysaccharides (LPS)-induced acute lung injury (ALI) mice. Methods: Eighteen beagle dogs were randomly divided into normal control, LPS alone, and LPS+naringin groups. Dogs in LPS+naringin group were pretreatment with naringin at dose of 12.4 mg/kg, while in LPS and normal control groups were dealt with placebos. 1 h latter, LPS (4 mg/kg) or saline was instilled intratracheally followed in 8 h of modeling period. Results: Naringin significantly decreased LPS-induced influx of inflammatory cells, the contents of total protein and cytokines production such as TNF-α and IL-8 in BAL fluid, while significantly but only partially inhibited the activities of MPO and iNOS in lung tissue. Moreover, naringin markedly attenuated LPS-induced increases total sputum volume, as well as pulmonary edema. Conclusion：pre-oral administration of naringin reduces inflammatory responses, pulmonary edema, sputum synthesis, and damage of lung tissue in LPS-induced ALI beagle do