脂日粮诱导的脑部氧化应激引起小鼠学习记忆障碍.docVIP

  高脂日粮诱导的脑部氧化应激引起小鼠学 习记忆障碍# 郑静1，李龙囡1，乐国伟1,2，施用晖1,2** 5 10 15 20 25 30 35 40 （1. 江南大学食品学院营养与安全研究所，无锡 214122； 2. 江南大学食品安全与科学教育部重点实验室，无锡 214122） 摘要：目的：探讨高脂膳食诱导的脑部氧化应激对小鼠学习记忆能力的影响及槲皮素的治疗 效果。方法：4 周龄 C57B/L 雌性小鼠 45 只，随机分为三组：对照组（基础日粮），高脂日 粮组（19.57%高脂日粮），槲皮素组（高脂日粮+0.01% 槲皮素），分别于 8 周和 26 周进 行 Morris 水迷宫实验，记录逃避潜伏期、原平台象限游泳时间与总时间之比、游泳路程与 总路程之比。实验结束后，取小鼠大脑皮层和海马组织测定谷胱甘肽比值（GSH/GSSG）、 总抗氧护能力（T-AOC）、丙二醛（MDA）、3-硝基酪氨酸（3-NT）和 4-羟基壬烯酸（4-HNE） 水平。结果：水迷宫试验中第 8 周小鼠各组间无显著差异，第 26 周高脂组小鼠学习记忆能 力显著下降（P0.05），槲皮素显著增强学习记忆能力；高脂组小鼠皮层 GSH/GSSG、T-AOC 以及海马 T-AOC 水平显著降低，皮层 MDA、3-NT 以及海马 MDA、4-HNE 含量显著升高 （P0.05），槲皮素显著减轻氧化损伤。结论：长期高脂饲喂诱导脑部氧化应激，引起蛋白 质和脂质氧化损伤，导致小鼠学习记忆能力减退，槲皮素减轻脑部氧化应激，增强学习记忆 能力。 关键词：高脂日粮 ；氧化应激；蛋白质氧化 ；脂质过氧化；学习记忆 ；槲皮素 中图分类号：R151 High-fat diet induced oxidative stress impairs memory and learning ability of mice Zheng Jing1, LI Longnan1, Le Guowei1,2, Shi Yonghui1,2 (1. JiangNan University, School of Food Science and Technology, The Institution of Food Nutrition and Safety, WuXi 214122; 2. The State Key Laboratory of Food Science and Technology, JiangNan University, WuXi 214122) Abstract: Objective To explore the effect of high-fat diet induced oxidative stress on memory and learning ability and the favorable effects of quercetin. Method Mice were randomly assigned to three groups: control group (normal diet), high-fat group (19.57%high-fat diet), Quercetin group (high-fat diet+0.01%quercetin) . Morris water maze experiments were apart conducted at 8th and 26th week ,then latency time , time and distance percentage in the platform quadrant were recorded. Mice were sacrificed after the water maze experiment at 26th week, then GSH/GSSG, T-AOC , MDA, 3-NT and 4-HNE in the cortex and hippocampus were tested. Result In water maze experiments, no significant difference was observed among the three groups at 8th week; the ability of learning and memory declined significantly at 26th week (P0.05), quercetin enhanced learning and memory significantly.Compared to control group, the level of GSH/GSSG ,T-