仿刺参(Apostichopus japonicus)配子发生过程中vasa基因在RNA及蛋白质水平上的表达特点.docVIP

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仿刺参(Apostichopus japonicus)配子发生过程中vasa基因在RNA及蛋白质水平上的表达特点.doc

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Expression Characteristics of vasa Gene at RNA and Protein Level in Apostichopus japonicus During Gametogenesis# 5 10 15 20 25 YAN Meng1, SUI Juan2, SHAO Mingyu1, SHENG Wanqiang3, ZHANG Zhifeng1** (1. Key Laboratory of Marine Genetics and Breeding, (Ocean University of China), Ministry of Education, Qingdao 266003; 2. Institute of Oceanology, Chinese Academy of Science; 3. Department of Biological Science, National University of Singapore) Abstract: The expression product of vasa gene is a widely conserved germ line marker and participates in the development regulation of reproductive system. However, no information on vasa gene in Holothuroidea is available. In this paper, we showed the expression characteristics of Apostichopus japonicus vasa gene (Aj-vasa) during gametogenesis in ovary and testis, using in situ hybridization and immunohistochemistry techniques. The expression of Aj-vasa RNA is strongest in early germ cells (oogoniums and spermatogonia), decreases as the maturation proceeded (oocyte and spermatocyte), and absents in spermatid and sperms. Our findings show that the overall expression trend of Aj-vasa RNA is similar with that of its protein（Aj-VASA） during gametogenesis. Nevertheless there was a sexuality difference about the subcellular location of vasa RNA and its protein which was reflected in the specifically expression in cytoplasm of female germ cells and the widely expression in both nucleus cytoplasm of male germ cells. It suggested that Aj-vasa RNA as well as Aj-VASA could be the marker of germ cells during gametogenesis. Key words: Apostichopus japonicus; vasa; gonad; gametogenesis; in situ hybridization; immunohistochemistry 0 Introduction Vasa gene has been recognized to be an excellent marker of germline among many species reflecting an essential function for germ cell development. The vasa gene was originally identified in Drosophila as a maternal-effect gene [1] and is present in germ cells throughout all [2] 30 35 eith