重组人骨形态发生蛋白2缓释微球的制备与评价.pdfVIP

中国组织工程研究与临床康复 第12 卷 第6 期 2008 –02 –05 出版 Journal of Clinical Rehabilitative Tissue Engineering Research February 5, 2008 Vol.12, No.6 基础实验 重组人骨形态发生蛋白2缓释微球的制备与评价*☆ 唐佩福，姚 琦，孙华燕，黄 鹏，崔 赓,王继芳 Preparation and evaluation of recombinant human bone morphogenetic protein-2 loaded microsphere Tang Pei-fu, Yao Qi, Sun Hua-yan, Huang Peng, Cui Geng, Wang Ji-fang Abstract Department of AIM: Recombinant human bone morphogenetic protein-2 (rhBMP-2) is prone to dilute and conduct metabolism, with a short Orthopaedics, Gen- half-life period in vivo. This study investigated the feasibility and the preparation of rhBMP-2 carrying poly (lactic-co-glycolide) eral Hospital of (PLGA), and observed drug-loading and drug-releasing function. Chinese PLA, Bei- METHODS: ①The rhBMP-2/PLGA microspheres were prepared using W/O/W solvent evaporation method. The particle size and j ing 100853, China morphology of the microspheres, the drug loading, the efficiency of encapsulation and in vitro release behaviors were detected.② PLGA microspheres containing 50 mg rhBMP-2 were implanted into medial vastus muscle pouches of right lateral thigh in 12 Tang Pei-fu ☆ , Kunming mice, and the ectopic bone formation and the activity of rhBMP-2 released from the microspheres were evaluated 4 weeks Doctor, Professor, Chief physician, later by the heterotopia bone formation experiment.