黄曲霉毒素B1与杂色曲霉素对HepG2细胞的联合毒性.docVIP

  黄曲霉毒素 B1 与杂色曲霉素对 HepG2 细胞 的联合毒性# 刘洋，张根义，杜明** 5 10 15 （江南大学食品学院） 摘要：目的 本文研究了自然界中常见的两种真菌毒素黄曲霉毒素 B1 (AFB1)和杂色曲霉素 (ST)，测定其对 HepG2 的细胞毒性及其联合毒性。方法 体外培养人肝癌细胞 HepG2，用不 同浓度梯度的 AFB1(1μM、5μM、10μM、50μM、100μM)和 ST(0.1μM、1μM、5μM、 10μM、14μM)单独和联合作用于 HepG2 细胞 24h 或 48h，通过分别测量细胞活性、ATP 含量、DNA 受损程度、线粒体膜电位以及活性氧含量的变化，来分析这两种毒素对 HepG2 细胞的联合毒性作用。结果 AFB1 和 ST 对 HepG2 细胞的 IC50 分别为 16.989μM 和 7.258 μM，两种毒素的相互作用指数 K=2.24, ATP、DNA、线粒体膜电位、活性氧等指标的理论 加和值与实际值相比，无显著性差异（P0.05），同时，2 种毒素及其混合物对 HepG2 细胞 的作用终点均可分为两类：第一类包括 ATP 含量、DNA 含量变化；第二类为活性氧含量变 化和线粒体膜通透性变化。结论 AFB1 和 ST 对 HepG2 细胞的联合毒性作用为加和作用。 关键词：细胞毒理学；黄曲霉毒素 B1；杂色曲霉素；细胞增殖力；毒性作用终点；联合作 用 中图分类号：TS207.3 20 Combinative Toxicity of Aflatoxin B1 and Sterigmatocystin to HepG2 LIU Yang, ZHANG Genyi, DU Ming (School of food sciencetechnology,Jiangnan University,Wuxi.214000) 25 30 35 Abstract: In this paper,Aflatoxin B1 (AFB1)and Sterigmatocystin(ST) are two common fungal toxins in nature, and we choose them to detect their joint cytotoxicity to HepG2. Human hepatoma cell line HepG2 was treated with different concentration of AFB1(1μM,5μM,10μM,50μM,100μ M)和 ST(0.1μM,1μM,5μM,10μM,14μM)for 24h or 48h to analyze the combinative toxic effect of the two toxins on HepG2 cells through measuring the change of cell activity, ATP content, degree of DNA damage, change of mitochondrial membrane potential and active oxygen content. We found that, the IC50 of AFB1 and ST is 16.989 μM and 7.258 μM,the interaction index is 2.24; there was no significant difference (P 0.05)between the theoretical value compared with the actual value of the indexes including ATP, DNA, mitochondrial membrane potential and active oxygen；at the same time,the five final points of cytotoxicity can be divided into two categories: the first category includes cell proliferation, ATP content, DNA content; the second class is the active oxygen content and the mitochondrial permeability transition.The results indicate that interaction type between AFB1 and ST is additive joint effect. Key words: