临床标本的真菌培育和药敏分析.pdfVIP

3·82 · Chin J Nosocomiol Vol. 13 No. 4 2003 ·论 　著· 临床标本的真菌培养和药敏分析 沈翠芬 , 张晓祥 (湖州市中心医院 , 浙江 湖州 313000) 摘要 : 目的 　研究本院真菌感染现状与耐药情况。方法 　各种临床标本经分离培养 ,用 A TB Expression 细菌鉴 定仪鉴定 ,采用 A TB FUN GUS 进行药敏试验。结果 　13 974 份临床标本共分离真菌 1 535 株 , 检出率为 109 % ;分离真菌 15 种 , 白色念珠菌仍为主要菌种; 各种真菌对 6 种抗真菌药物均出现了不同程度的耐药。 结论 　真菌感染在临床上呈上升趋势且产生了耐药株 ,临床微生物实验室不仅应做好真菌的分离培养鉴定 ,而且 要对分离的菌株进行药敏试验 , 以指导临床合理选择抗真菌药物。 关键词 : 真菌 ; 药敏试验 中图分类号: R379 　　文献标识码 : A 　　文章编号 : (2003) Fungus Culture and Antif ungal Sensitivity : Analysis of Clinical Samples SHEN Cuifen , ZHAN G Xiaoxiang ( ) Depart ment of L aboratory Medicine , Huz hou Cent ral Hospital , Huz hou 313000 , China Abstract : OBJECTIVE To investigate the characteristics of fungus infection and antifungal resistance at Huzhou Central Hospital in Huzhou , Zhejian g Province. METHODS The fungi were cultured , isolated and identified with A TB expres sion microbe identification system. The antifungal sensitivity was detected with A TB FUN GUS system . RESULTS A total of 1 535 strains of fungi were isolated from 13 974 samples , the isolation rate was 10. 9 %. There were 15 species of fungi having been identified all together , Candida albicans was still of the main one. Each species was found to be resistant to 6 antifungals in various degree. CONCL USIONS Fungus infection rate raised gradually and the antifungal re sistant strains were found from time to time. In order to guide clinically the selection of the reasonable antifungals , the isolation and ide