参附注射液对病毒性心肌炎模型小鼠心肌腺苷酸酶活性和钠／钙代谢的影响.pdf

参附注射液对病毒性心肌炎模型小鼠心肌腺苷酸酶活性和钠／钙代谢的影8向△ 刘 琼 ，陈建斌 ，饶邦复 (重庆医科大学附属第一医院急诊科，重庆市 400016；重庆医科大学附属第一医 院心内科，重庆市 400016) 中图分类号 R605；R974 文献标识码 A 文章编号 1001—04082【009)33—2570—03 摘 要 目的：研究参附注射液对病毒性心肌炎模型小鼠心肌腺苷酸酶活性及钠、钙代谢的影响。方法：实验分为4组，即对照、感 染及参附注射液高、低剂量组。在不同时相点分别测定小鼠心肌腺苷酸酶活性和钠 ／钙含量，观察组织病理变化。结果：与对照组比 较，感染组心肌Na ，K 一ATPase及 Ca 一ATPase活性显著减弱，钠、钙含量显著升高 (P0．01)，并早于心肌结构变化；与感 染组比较，参附注射液高剂量组Na，K ATPase及Ca 一ATPase活性显著增强，钠、钙含量显著降低 (P0．01或P0．05)，且 心肌炎症、坏死程度也显著减轻(PO．01或P0．05)。结论：参附注射液能显著改善模型小鼠病毒感染后心肌继发性损伤状况，其机 制可能与抑制心肌腺苷酸酶活性下降，阻止反常性钠、钙超载有关。 关键词 病毒性心肌炎；腺苷酸酶；钠；钙；参附注射液 EffectsofShenfuInjectiononMyocardialATPaseActivitiesandSodium／Calcium Metabolism inViral M yocarditisModelM ice LIUQiong(Dept．ofEmergency，TheFirstAffiliatedHospitalofChongqingMedicalUniversity．Chongqing 400016，China) CHEN Jian—bin，RAOBang—fu(Dept．ofCardiology，TheFirstAffiliatedHospitalofChongqingMedicalU— niversity，Chongqing400016，China) ABSTRACT OBJECTIVE：ToexploretheeffectsofShenfuinjectiononmyocardialATPaseactivitiesandsodium／calcium metabolism inviralmyocarditismodelmice．METHODS：Theexperimentalmicewererandomlydividedinto4groups：control group，infectedgroup，highdoseShenfuinjectiongroupandlow doseShenfuinjectiongroup．ThemyocardialATPaseactivi— tiesandconcentrationsofsodium ／calciumweredeterminedatdifferenttimepoins，andthepathologicchangeswereinvestigated aswel1．RESULTS：Ascomparedtocontrolgroup，theactivitiesofNa ，K 一ATPaseandCa ATPaseoftheinfected groupdecreasdsignificantly (P0．01)，buttheconcentrationsofsodium andcalcium increasedmarkedly (P0．01)，and thesechangesoccurredearlierthanhistologicabnormalitiesofcardiacmuscle．InhighdoseShenfuinjectiongroupascompared totheinfectedgroup．theactivitiesofNa ．K 一ATPaseandCa”一ATPasewereenhancedsignificantlyandtheconcentra- tionsofsodium andcalcium decreasedmarkedly(P0．01orP0．05)．andtheimprovementofmyocarditisandmyocytes necrosisweremoresignificant(P0．01orP0．05) ．CONCLUSION：TheremarkableefficacyofShenfuinjectioninallevi— ati