TGF-β1 inhibits connexin-43 expression in cultured smooth muscle cells of human bladder.pdf

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2017-09-13 发布于湖北
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TGF-β1 inhibits connexin-43 expression in cultured smooth muscle cells of human bladder.pdf

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AvailableonlineatWWW．sciencedirect．corn JOURNALOF MEDICAL ScienceDirect COLLEGESOFPLA JournalofMedicalCollegesofPLA24(2009)283—287 www．elsevier．corn／locate／jmcpla TGF--Plinhibitsconnexin-·43expressioninculturedsmooth mUuSsCclleecceellllsso工fhumanbladder ChiQiang，ZhouFenghai米，WangYangmin DepartmentofUrology．LanzhouGeneralHospitalofPLA．Lanzhou730050,China Received9April2009；accepted26June2009 Abstraet Objective：Inthisresearch，westudiedtheTGF—p1effectsonconnexin一43expressioninculturedhumanbladdersmooth musclecells．Methods：Human bladdersmooth muscle cellsprimary culutres，with bladdertissue obtained from patients undergoingcystectomy,wereintervenedbyrecombinanthumanTGF—p1．Connexin一43expressioninhumanbladdersmooth musclecellswasthen examined by Western blotting and immunocytochemistry．Results：Stimulation with TGF一3lled to significantreduction ofconnexin一43 immunoreactivity and coupling fPO．ooo1)．Cormexin一43 protein expression was significantlydownregulated(P0．o5)．Simultaneously,lowphosphorylationspeciesofconnexin一43wereparticularlyaffected． Conclusion：Ourexperimentsdemonstratedasignificantdownregulationofconnexin一43byTGF—B1inculturedhuman bladdersmoothmusclecells．Thesefindingssupporttheview thatTGF—p1isinvolvedinthepathophysiologyofurinary bladderdysfunction． Keywords：Connexin；Cytokines；Urinarybladder 1．Intr0ducti0n showninthehyperactiveratbladderfollowingpartial