具有广谱抑菌活性的植病生防菌株JK-2的筛选和鉴定.docVIP

具有广谱抑菌活性的植病生防菌株JK-2的筛选和鉴定( 葛慈斌1，郝晓娟1、2，黄素芳1，朱育菁1，郑雪芳1，刘波1* （1.福建省农业科学院生物技术研究所生物农药研究中心，福州 350003； 2.浙江大学农业与生物技术学院，杭州 310029） 摘要：从福建省永泰、福清、闽侯等6个县（市）采集作物根际土壤，筛选出芽孢杆菌58株。采用目标病原菌多重菌株平行测定法，试验初筛出的芽孢杆菌对6株枯萎病尖孢镰刀菌的抑制作用，筛选得到拮抗菌株3株，其中1株（定名为JK-2）的抑菌效果特别显著。对JK-2的抑菌谱调查结果表明，该菌株对大丽轮枝菌（Verticillium dahliae）、黑白轮枝菌（Verticillium albo-atrum）、真菌轮枝霉Verticillium fungicola）、番茄灰霉病原菌（Botrytis cinerea）、青枯雷尔氏菌（Ralstonia solanacearum）和禽致病性大肠杆菌（Escherichia coli）都具有较强的抑制作用。对JK-2进行菌体形态、培养特征观察及生理生化试验，再结合16S rDNA序列测定及Sherlock MIS脂肪酸分析，判定该菌株为短芽孢杆菌（Brevibacillus brevis）。将JK-2培养液浇灌移栽后1天的西瓜苗，试验其田间防治西瓜枯萎病的效果，1个月后，防效可达78.26%。 关键词：生防菌；尖孢镰刀菌；短芽孢杆菌；Sherlock MIS脂肪酸分析 中图分类号： 文献标识码：A Screening and Identification of Biocontrol Bacteria Strain JK-2 against Plant Pathogenic Fungi and Bacteria GE Ci-bin1, Hao Xiao-juan1,2, Huang Su-fang1, ZHU Yu-jing1, Zheng Xue-fang1,LIU Bo1* (1 Biocide Center，Institute of Biotechonology, FAAS, Fuzhou 350003，China; 2.Institute of Biotechonology，University of Zhejiang，Hanzhou 310027，China) Abstract: Fifty-eight strains of Bacillus spp. were isolated from the rhizosphere soils, which were collected from Yongtai, Fuqing, Minhou and other 3 counties in Fujian Province. Using multiple target pathogens inhibiting test with 6 strains of Fusarium oxysporum Schl., three strains of biocontrol bacteria were screened to show high inhibition against F. oxysporum. Among these strains, one strain, which was named with JK-2, was the most effective. JK-2 had strong antagonistic effect on a broad spectrum of pathogenic fungi and bacteria, such as Verticillium dahliae, Verticillium albo-atrum, Verticillium fungicola, Botrytis cinerea, Ralstonia solanacearum and Escherichia coli. Based on the observations on its morphological features and culture characters, a series of physiological and biochemical experiments, Sherlock MIS analysis and its 16S rDNA sequence phylogenetic relation, JK-2 was identified as Brevibacillus brevis. The bioassay in field indicated that JK