混合菌对石油的降解及其降解性能的研究.pdfVIP

330 第三届全国农业环境科学学术研讨会论文集 2009 年 10 月 混合菌对石油的降解及其降解性能的研究 何丽媛 1,2 ，党 志 1,2 （1.华南理工大学环境科学与工程学院，广东 广州 510006；2.教育部工业聚集区污染控制与生态修复教育部重点实 验室，广东 广州 510006） 摘 要：分别从土壤中分离筛选到能降解烷烃的洋葱伯克霍尔德氏菌(Burkholderia cepacia)GS3C、降解菲的鞘氨醇单 胞菌(Sphingomonas sp.)GY2B、降解芘的假单胞菌(Pseudomonas sp.)GP3A 和伯克菌科的菌株 Pandoraea pnomenusa GP3B 经驯化后都对石油有一定的降解效果。本研究将这些菌株正交混合，观察混合菌对石油的降解情况，通过气相色谱- 质谱联用法（GC-MS）分析混合菌对不同石油组分的降解作用，并研究混合菌的降解性能。结果表明，混合菌 G8 （GS3C+GY2B+GP3B）对石油的去除率比单菌株提高了近 30%，且对石油中的烷烃类和芳烃类化合物都有较强的降 解能力，培养 5 d 后使初始原油浓度为 2 000 mg ·L-1 的去除率达到 69.20%，其最适生长条件为：温度为 30 ℃，初始 pH 值为 7，接种量为 4%。 关键词：混合菌；石油；生物降解；GC-MS Biodegradation Characteristics of Petroleum by Mixed Strains 1,2 1,2 HE Li-yuan ，DANG Zhi (1. School of Environmental Science and Engineering, 2.South China University of Technology; 2. The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters (Ministry of Education), Guangzhou, 510006,China) Abstract: Four petroleum component degrading strains including alkane-degrading strain Burkholderia cepacia GS3C, phenanthrene-degrading strain Sphingomonas sp. GY2B, pyrene-degrading strain Pseudomonas sp. GP3A and Pandoraea pnomenusa GP3B,isolated from soil had petroleum degradation effect after domestication using petroleum as the sole carbon source. This study mixed the four strains by quadrature, and studied the petroleum degradation characteristics of the mixed strains by using gas chromatography and mass spectrometry (GC-MS). The results show that the mixed strains G8 (GS3C+GY2B+GP3B) had enhanced the petroleum removal rate nearly 30% than the individual strains, and had a strong degradation ability on both alkanes and aromatic hydrocarbons. It could d