piggyBac转座系统在哺乳动物及其细胞中的研究进展.pdfVIP

第23卷第3期 生命科学 Vol. 23, No. 3 2011年3月 Chinese Bulletin of Life Sciences Mar., 2011 文章编号：1004-0374(2011)03-0255-06 piggyBac转座系统在哺乳动物及其细胞中的研究进展 1,2 1,2 1,2 * 谢　飞 ，蒋世忠 ，马晴雯 (1 上海市儿童医院医学遗传研究所，上海 200040 ；2 上海交通大学医学院，上海 200025) 摘　要：piggyBac(PB)转座系统来源于昆虫鳞翅目，属于真核生物的第二类转座系统，主要采取 “剪切粘 帖”机制发生转座。PB系统转座效率高，宿主范围广，广泛应用于昆虫等低等生物的基因转移及突变筛 选。近年来，研究发现PB系统在哺乳动物及其细胞中也具有高效的转座活性，已在动物基因组功能研究、 基因转移及诱导多能干细胞等领域得到了广泛应用。本文就PB系统近年来在哺乳动物及其细胞中的研究进 展、应用前景及存在问题进行了综述。 关键词：piggyBac ；转座；哺乳动物；转基因 中图分类号：Q78 文献标识码：A Current advance of piggyBac in mammals and mammalian cells XIE Fei1,2, JIANG Shi-Zhong1,2, MA Qing-Wen1,2 * (1 Institution of Medical Genetics of Shanghai Children’s Hospital, Shanghai 200040, China; 2 Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China) Abstract: piggyBac (PB) transposon, a TTAA-specific transposon firstly isolated from the genome of cabbage looper moth, is a member of class II transposon family, which jumps via a “cut and paste” mechanism. PB transposon has been widely used for insertion mutagenesis and carrying gene in lower organism. Recent studies demonstrate that PB transposon system possesses high activity of delivering large pieces of DNA in mammals and cultured mammalian cells and can remobilize efficiently without any track. These new findings accelerate the investigation about PB transposon system in transgenesis, gene therapy, functional genomics and generation of induced pluripotent stem cell (iPSC). In this review, we summarized the recent progresses of PB in mammalian and cultured mammalian cells, and discussed the obstacles in application