广西番茄晚疫病菌交配型鉴定及生理小种的初步检测.pdfVIP

维普资讯 · 266 · 广西农业科 学 2007年第 38卷第 3期 M atingtypeidentification andpreliminaryphysiologicalracetest ofPhytophthorainfestansfrom tomatoinGuangxi ZHU Gui—ning，HUANG Fu—xin，FENGLan—xiang，YANGYu—hong，QinBi—xia (1．PlantProtectionResearchInstitute，GuangxiAcademyofAgriculturalSciences，Nanning530007，China； 2．InstituteofVegetableandFlowers，ChinaAcademyofAgriculturalSciences，Beijing 100081，China) Abstract：During2000—2006，508samplesoftomatolateblightPytophthorain／stansfrom 8tomatogrowing areasinGuangxiwerecollectedand239pureisolatesofthepathogenwereobtained．Theresultsofmatingtype testsshowedthatAlwasthemainmatingtypeinGuangxi，whichaccountedfor96．65 ofthetotalisolates，and 8isolatesbelongedtoAzmatingtypewhichwerefoundinTianyang，Tiandong，W um ingandLiuzhou．Byusing sixdifferentialtomatohostspossessingdifferentresistancegenes，i．e．Tsl9，Ts33，W ．Va700，CLN2037B，L3708 andLA1033，fiveracesT0，Tl，T1．z，T1．z．4andT1．z．3．4．5wereevaluatedamongthe32isolatesselectedfrom 7a卜 easforthepreliminaryphysiologicalracetests．Amongthem ，racesT1．zandT1．z．3．4．5werepredominant，widely distributing in 5 and 3areaswith high frequenciesof37．50 and 34．37 ，respectively，and raceT0and Tldistributedin 5and 3areaswiththefrequenciesof 15．63 and 9．37 ，respectively，whileraceTllz．4was foundonlyinTianyangwiththefrequencyof3．13 ．TheresultsindicatedthatthecharacteristicsofP．infestarts populationsontomatoinGuangxiwerecomplicatedanddiverse． Keywords：Phytophthorainfestans；matingtype；physiologicalrace；differentialhost；identification CLC number：$436．412．1 2 Documentcode：A ArticleID：lOO2— 8161(2007)03—0266— 05 广西番茄晚疫病菌交配型鉴定及生理小种的初步检测 朱桂宁 ， 黄福新 ， 冯兰香 ， 杨宇红 ， 秦碧霞 (1．广西农业科学院植物保护研究所 ， 南宁 530007； 2．中国农业科学院蔬菜花卉研究所，北京 100081)