马氏珠母贝黄壳色选育系G1遗传结构的ISSR分析.pdfVIP

第 29 卷 第 1 期 广东海洋大学学报 Vol.29 No.1 2009 年 2 月 Journal of Guangdong Ocean University Feb. 2009 马氏珠母贝黄壳色选育系 G1 遗传结构的 ISSR 分析 郭 俊，邓岳文，杜晓东，王庆恒 （广东海洋大学水产学院，广东 湛江 524025） 摘 要：挑选 11 个黄壳色的马氏珠母贝养殖个体为亲本繁殖子代群体，并随机选取 50 个体为亲本，建立对照群体。 采用 ISSR 分子标记对马氏珠母贝黄壳色群体和对照群体的遗传结构差异进行分析。结果表明，黄壳色和对照群体 的遗传多样性分别为 0.358 7 和 0.373 3 ，多态位点比例分别为 86.89％和 90.16％；群体间遗传分化系数为 3.75％。 可见，经过一代选择后，黄壳色群体遗传结构指标略低于对照群体，但两个群体未发生明显分化。 关键词：马氏珠母贝，黄壳色选系，遗传结构，ISSR + 中图分类号：Q75 + Q959.215 .4 文献标志码：A 文章编号：1673-9159（2009）01-0087-04 Genetic Structure of Yellow Shell Color Line of Pearl Oyster Pinctada martensii Based on ISSR Marker GUO Jun, DENG Yue-wen, DU Xiao-dong, WANG Qing-heng (Fisheries College, Guangdong Ocean University, Zhanjiang 524025, China ) Abstract: The first generation selected group was established by selecting breeders with yellow shell color in the base population of pearl oyster Pinctada martensii . A control group was also obtained by selecting mature individuals in the same base population as breeders. The genetic diversity values of the selected and control groups were compared by the inter-simple sequence repeats marker (ISSR). The Shannon genetic diversity values of the selected and control groups were 0.3587 and 0.3733, respectively. The polymorphic locus percentages were 86.89 % and 90.16%, respectively. The genetic differentiation between the two groups was 3.75%. It is suggested that there was no significant genetic differentiation between the two groups af