荧光定量PCR方法检测畜肉食品中猪源性成分.pdfVIP

4 5 Vol. 4 No. 5 2013 10 Journal of Food Safety and Quality Oct. , 2013 PCR 1* 1 2 1 1 王 颖 , 史艳宇 , 刘金华 , 吕 航 , 王 莹 (1. , 130022; 2. , 130062) 摘 要: 目的 方法 12S rRNA , PCR , ; ; 50 mg/kg DNA DNA , 结果 , , ( 0.1 μg/kg)结论 , , 关键词: ; 12S rRNA ; real time PCR; Detection of porcine-derived materials in meat products by real time PCR method 1* 1 2 1 1 WANG Ying , SHI Yan-Yu , LIU Jin-Hua , Lv Hang , WANG Ying (1. Jilin Product Quality Supervision Inspection, Changchun 130022, China; 2. Jilin Entry-Exit Inspection and Quarant Bureau, Changchun 130062, China) ABSTRACT: Objective To establish a rapid, specific, sensitive detection method for porcine derived mate- rials. Methods A real-time PCR method for the porcine derived materials detection in meat products was es- tablished using forward and reverse primers and probe corresponding to the mitochondrial 12S rRNA gene. The specific detection was carried out by livestock and poultry meat including lamb, beef, chicken, goose, duck, rabbit meat, horse meat, venison and other reference animal species and 50 mg/kg lamb DNA was chosen as diluent by gradient dilution of pork DNA, then sensitivity detection was executed. Results This method could effectively detect the porcine-derived materials, and showed a strong specificity and high s