则明显地延后,此现象需要更多的实验才能有较适当的模型产.pdfVIP

摘要 暗適應的葉片突然被強激發光照射後，會產生多階段的螢光上升 (O-J-I-P polyphasic fluorescence rise) 。對此一現象的解釋目前大家並沒有共識，但已將它廣泛地運用於光合作 用系統的分析上。本計畫對 polyphasic fluorescence rise做了較深入的探討。我們利用只有 兩層細胞的水蘊草超薄葉片為材料，證實了OJIP rise是光系統的固有性質，而非因葉片的 特殊透光性而產生的螢光遲滯。另外也只有J phase會對激發光的強度和第二光系統 OEC (oxygen evolving complex)的 S states 產生反應，似乎顯示J phase (為一光化學反應)與I 、P phases的成因並不是屬於同一類。當溫度下降至 0°C時，J phase會一分為二，而 I和 P phases 則明顯地延後，此現象需要更多的實驗才能有較適當的模型產生。 When the dark adapted leaf is illuminated by a strong actinic light, it emits fluorescence that rises with multi-steps (O-J-I-P rise). There is still no consensus in explanation, but it has already widely employed in characterization of photosynthetic system. In this project, we made further study on the OJIP rise, using Egeria densa, whose leaves are only two-cell thick, as experimental materials. It was found that OJIP rise is an intrinsic property of photosystem II, but not fluorescence delay caused by light penetration problem of leaves. We also found that only J phase responses to actinic light intensity and the S state of photosystem II oxygen evolving complex, suggesting that the origin of J phase (a photochemical process) is different from those of I and P phases. In addition, when temperature is dropped to 0°C, the J phase splits into two, whereas I and P phases delay significantly. More experiments are required to generate a suitable model. 關鍵詞 葉綠素螢光, 多階層螢光上升, 水蘊草 ,第二光系統 , 光合作用 Key words: chlorophyll fluorescence, polyphasic fluorescence rise, Egeria densa, phot