聚乙二醇增敏-流动注射光度法测定微量铅.pdfVIP

聚乙二醇增敏-流动注射光度法测定微量铅 * 龚爱琴，朱霞石 , 黄晓艳, 张亚琴 扬州大学化学化工学院，扬州，225002 摘要：本文提出了聚乙二醇增敏-流动注射法测定药品中微量铅的新方法。在聚 2+ 乙二醇-800（PVA-800）介质中，以pH10.0 的硼砂-NaOH为缓冲溶液，Pb 与 5-Br-PADAP形成络合物。考察了影响测定的主要影响因素。试验结果表明: 在 2+ 优化条件下，Pb 浓度在 0.0～7.0μg/25mL范围内符合比尔定律， A=-0.005+0.60 c(c: μg/mL),r=0.9996,检出限为1.5n g/mL,检测速度为10/h， 同时考察了共存离子的干扰并做了回收试验。本法用于测定中标准水样及实际样 品药葶苈子，黄连中的铅含量，结果满意。 关键词：铅， 5-Br-PADAP，流动注射光度法 中图分类号：O482.8 文献标识码：A THE DETERMINATION OF TRACE LEAD BY FLOW INJECTION ANALYSIS IN POLYETHYLENEGLYCOL MEDIUM Abstract: In this work, a new flow injection analysis (FIA) for the determination of Pb2+ in Chinese medicinal herbs was developed. In the buffer solution of borax- NaOH (pH=10.5), Pb2+ reacted with 2-[(5-Bromo-2-Pyridyl) -azo]- 5- (diethl- amino) phenol (5-Br-PADAP) to form a complex. The experimental results showed that the sensitivity was enhanced in the present of polyethyleneglycol-800(PG-800). The main factors affecting the determination were investigated in details. Under the optimum conditions, the liner range and detection limit is 0.0 to 0.3μg/mL and 1.5ng/mL (correlation coefficient r = 0.9996), respectively. The linear regression equation is A=-0.005+0.60c (μg/mL). The sample throughout is 10/h. Foreign substrates effects were also investigated. The proposed method has been successfully applied to the determination of lead in reference material, goldthread and lepidium seed. Keywords: lead; FIA; polyethyleneglycol-800 * Corresponding author. Tel/Fax:+86-514-7975244 E-mail address: xszhu@; zhuxiashi@ 1.引言 铅是对人体有害的元素，它易在人体内积蓄引起慢性中毒，其生