端粒酶、P16、RB、PCNA在胰腺癌组织中的表达及意义.DOCVIP

临床医学论文-端粒酶、P16、RB、PCNA在胰腺癌组织中的表达及意义 ?????????? 作者：许元鸿 欧阳兵 于国志 郭仁宣 郭克建? 【摘要】? 目的 探讨端粒酶活性、P16、RB、增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)在胰腺癌组织中的表达及意义。方法 对39例胰腺癌及11例胰腺良性病变采用PCRTRAP方法检测端粒酶活性，免疫组化SP法定位观察P16、RB、PCNA表达。结果 端粒酶、P16、RB、PCNA标记指数(labeling index,LI)在胰腺癌中表达阳性率分别为92.3%(36/39)、43.6%(17/39)、71.9%(28/39)、(47.8±11.3)%。与胰腺良性病变比较差异有统计学意义(P0.05)。端粒酶阳性、P16和RB表达缺失者PCNA LI显著增高(P0.05)。端粒酶活性与P16表达、P16与RB表达均呈显著负相关(P0.05)。P16/RB失活组端粒酶阳性率显著高于P16阳性/RB阳性组(P0.05)。P16表达缺失和PCNA高值组临床病期较晚，更易发生淋巴结转移(P0.05)。结论 端粒酶激活、P16、RB表达缺失在胰腺癌发生中起重要的调节作用，而且是促进细胞增殖重要因素之一。P16表达缺失与胰腺癌的转移和浸润可能有密切关系。 【关键词】? 胰腺肿瘤； 端粒酶； P16； RB； PCNA； 基因 ????? 【Abstract】? Objective? To investigate expressions of significance of telomerase, P16, RB and PCNA in pancreatic cancer tissues and their significance. Methods?Expressions of P16, RB and PCNA in tissues from 39 cases with pancreatic cancer and 11 benign leisions were observed by means of immunohistochemical method. In the meantime, telomerase activity was investigated by using telomeric repeat amplification protocal (TRAP) assay. Results?The positive expression rate of telomerase, P16, RB, PCNAL1 in pancreatic cancer tissues were 92.3%, 43.6%, 71.9% and (47.8?1.3)%, respectively, with statistica difference compared with benign leisions (P0.05 or P0.01). The cases with expression deletion of telomerase activation, P16 and RB showed significanty higher level level PCNA LI （P0.05）. There found negative correlation between expression of? P16 and that of RB as well as expression of telomerase and that of P16 (P0.05). The positive rate of telomerase in P16/RB deletion group was significantly higher than that in P16+/RB+ group (P0.05). The expression deletion of P16 and high level of PCNA were correlated closely with lymph node metastasis (P0.05). Conclusions?Telomerase activation, expression deletion of P16 and RB genes may be important molecular event in occurrence of pancreatic cancer and is one of vital factors pomoting cell proliferation. The loss express