肺缺血再灌注损伤时一氧化碳的变化及葛根素对其影响.DOCVIP

临床医学论文-肺缺血再灌注损伤时一氧化碳的变化及葛根素对其影响 【摘要】? 目的:观察兔肺缺血再灌注损伤时一氧化碳的变化及葛根素对其的影响。方法:30只健康日本大耳白兔随机分成假手术对照组（C组）、肺缺血再灌注组（IR组）和葛根素组（Pur组）。复制兔单侧肺缺血再灌注损伤模型，各组在缺血前、缺血1 h、再灌注1 h、3 h和5 h分别抽血，检测一氧化碳血红蛋白(COHb)浓度、环磷酸鸟苷(cGMP)含量。实验结束时取肺组织检测cGMP含量，电镜观察肺组织超微结构的改变。结果:血浆COHb浓度、cGMP 含量和肺组织cGMP 含量IR组、Pur组明显高于C组，以Pur组为著（P0.01）。电镜显示IR组有明显的肺超微结构损伤，而Pur组损伤较轻。结论:葛根素可通过提高内源性CO水平，对缺血再灌注损伤肺组织发挥保护作用。 【关键词】? 肺 缺血再灌注损伤 一氧化碳 环磷酸鸟苷 葛根素 ??? Abstract:? Objective:To study variations of carbon monoxide in lung ischemia-reperfusion injury and effects of puerarin. Methods:The unilateral lung ischemia-reperfusion model was replicated in vivo. Rabbits were randomly divided into three groups (n=10 in each), control (C) group, ischemia-reperfusion (IR) group and puerarin (Pur) group. The blood specimens gathered before ischemia, ischemia 1 h, reperfusion 1 h, 3 h and 5 h were tested for the content of carboxyhemoglobin (COHb), cyclic guanosine monophosphate(cGMP). The lung tissues sampled at 5 hours after reperfusion were assayed for the content of cGMP, the ultrastructural changes were observed under electron microscope. Results:The plasma content of COHb, the content of cGMP of plasma and lung tissue in IR and Pur group were much higher than that in C group, and the index in Pur group increased more significantly（P 0.01）. Severe ultrastructural morphological damage in IR group was observed under eletron microscope, but lightly injury was found in Pur group. Conclusion:Puerarin provides significant protective effects on lung during ischemia-reperfusion injury through increasing the plasma content of COHb. ??? Key words:?? lung; reperfusion injury; carbon monoxide; cyclic guanosine monophosphate; puerarin ??? 肺缺血再灌注损伤（Lung ischemia reperfusion injury，LIRI）是肺溶栓及肺移植影响预后的一个重要因素，因为LIRI约20%的肺移植患者会出现致死性的移植物功能不全，也是术后一年15%的死亡率的主要原因之一，故对LIRI进行干预、减轻损伤是当前国内外的研究热点。一氧化碳（CO）是具有舒张血管、抗血小板聚集、抑制血管平滑肌细胞增生和血管内皮细胞凋亡等作用的细胞信使分子[1]。本实验通过观察LIRI时CO水平的变化及葛根素对它的影响，以探讨CO在LIRI中的作用及葛根素防治LIRI的机制。 ??? 1? 材料和方法 ??? 1.1 材料? 健康日本大耳白兔30只，雌雄不拘，体重1.7～2.7