肝细胞癌组织芯片环氧合酶-2表达的半定量图像分析.DOCVIP

临床医学论文-肝细胞癌组织芯片环氧合酶-2表达的半定量图像分析 【摘要】? 目的 分析环氧合酶-2(COX-2)在肝细胞癌(HCC)中的表达及意义。 方法 应用改良的手工制备组织芯片方法制备含150例肝细胞癌、92例癌旁肝硬化、45例癌旁慢性肝炎以及37例肝内转移灶和13例正常肝组织的组织芯片,结合免疫组织化学染色和图像分析技术检测COX-2蛋白的相对表达量。 结果 COX-2蛋白在HCC表达显著低于癌旁肝硬化、癌旁慢性肝炎(P0.01)，但高于正常肝组织(P0.05),差别具有统计学意义。在HCC组织中,低分化HCC的COX-2表达量显著低于高分化的HCC,差别有统计学意义(P0.01)。COX-2蛋白的表达与性别、肿瘤大小、组织学类型、包膜侵犯及转移均无关(P0.05)。 结论 COX-2在HCC的发生中发挥重要作用,并与HCC的分化程度密切相关,但与HCC进展及侵袭转移关系不大。 【关键词】? 前列腺素内过氧化物合酶; 肝肿瘤; 癌,肝细胞; 芯片分析技术; 免疫组织化学; 图像处理,计算机辅助 ??? ABSTRACT:? Objective? To detect the expression of cyclooxygenase-2(COX-2) in hepatocellular carcinoma(HCC).?Methods?Tissue chips were constructed by modified manual tools, which contained 15别有0 HCC samples, 92 cirrhotic livers adjacent to HCC samples, 45 chronic hepatitis livers adjacent to HCC samples, 13 normal liver tissue samples and 37 intrahepatic metastasis lesion samples.?The expression of COX-2 was determined by immunohistochemistry and semi-quantitative image analysis.?Results?The expression of COX-2 in HCC was lower than that in cirrhotic liver adjacent to HCC tissue or chronic hepatitis tissue, but higher than that in normal liver tissues(P0.01).?More lower differentiation of HCC more the expression of COX-2 decreased. COX-2 expression was not correlated with sex, age, tumor size, histological type, tumor invasion and metastasis(P0.05).?Conclusion?COX-2 might play an important role in the process of HCC tumor genesis and the differentiation of HCC, but not related with invasion and metastasis. 牋?KEY WORDS:?prostaglandin-endoperoxide synthase; liver neoplasm; carcinoma,hepatocellular; microchip analytieal procedures; immunohistochemistry; image processing,computer-assisted ??? 计算机图像处理系统的诞生是计算机技术与形态影像为主的学科相结合的结果,使得定量病理学得到长足发展。目前,彩色图像分析系统已较广泛应用于免疫组织化学结果的分析中［1］。福建省是肝细胞癌(hepatocellular carcinoma,HCC)高发的省份。笔者应用组织芯片和图像分析技术检测环氧合酶-2(cyclooxygenase-2,COX-2)在HCC的表达情况,探讨其与各临床病理参数的关系及意义。 ??? 1? 材料与方法 ??? 1.1? 对象? 收集福建医科大学附属第一医院2000—2006年手术切除的HCC标本150例,癌旁肝硬化92例,癌旁慢性肝炎45例,肝内转移灶(卫星灶和门静脉癌栓)37例。所有病例均经病理诊断证实,