涎腺肿瘤中HSP27的表达特征及与p53、PCNA的相关性研究.DOCVIP

临床医学论文-涎腺肿瘤中HSP27的表达特征及与p53、PCNA的相关性研究 ????????????????? 作者：顾晓林 王桂兰 曹斌 陈莉 鄂群 【摘要】? 目的：分别研究热休克蛋白27（heat shock protein 27，HSP27）在涎腺良性、恶性肿瘤中的表达特征及其与抑癌基因p53和增殖细胞核抗原（proliferating cell nuclear antigen，PCNA）的相关性，探讨其在涎腺肿瘤发生发展中的影响和意义。方法：采用免疫组化法检测41例良性和17例恶性肿瘤中HSP27、p53、PCNA的表达。结果：（1）HSP27阳性表达于肿瘤细胞浆，p53、PCNA胞核表达为主；（2）HSP27在良性肿瘤中高表达，在恶性肿瘤中表达下降，HSP27的表达与涎腺肿瘤细胞的分化程度显著相关（P0.01）；（3）p53、PCNA在恶性肿瘤的表达均显著高于良性肿瘤。HSP27和p53、PCNA有一定反向趋势（P＞0.05）。结论：HSP27表达减弱可能是涎腺恶性肿瘤分化降低的标记；HSP27的表达减少、PCNA阳性和p53突变可能协同促进恶性肿瘤细胞的增殖。 【关键词】? 涎腺肿瘤；热休克蛋白27；p53；增殖细胞核抗原；免疫组化 ??? [Abstract]?? Objective: To study the expression characteristics of heat shock protein（HSP） 27 in salivary benign and malignant tumors and their correlation with tumor suppressor gene p53 and PCNA，and to investigate their effects on occurrence and development of salivary gland tumors. Methods: Immunohistochemistry methods were used to detect the expressions of HSP27，p53，PCNA in 41 cases of benign tumors and 17 cases of malignant tumors. Results:（1）HSP27 positive expression mainly located in the cytoplasm of tumor-epithelial cells，and p53，PCNA expression mainly in nucleus; （2）The expression of HSP27 was high in benign tumors than that in malignant tumors，which was correlated with the differentiation of tumor cells（P0.01）；（3）p53，PCNA expressions in malignant tumors were significantly higher than those in benign tumors. There was a certain reverse trend between the expressions of HSP27 and p53 or PCNA（P0.05）. Conclusions: The lower expression of HSP27 may be the biomarker of differentiation depression. Reduced expression of HSP27，positive PCNA and mutation of p53 may co-promote proliferation of malignant tumor cell. ??? [Key words]?? Salivary tumor；Heat shock protein 27；p53；Proliferating cell nuclear antigen；Immunohistochemistry ??? 　　热休克蛋白27（heat shock protein 27，HSP27）是一种小分子热休克蛋白，是生物细胞受刺激后，选择性合成的一类高度保守的蛋白质，普遍存在于所有生物界原核细胞和真核细胞的细胞膜、细胞质及细胞核内，能潜在贡献于膜完整性的维持，尤其在刺激条件下[1]。近年来研究发现HSP27与多种肿瘤的生物学行为、治疗及预后有密切关系[2-4]。但其在涎腺肿瘤中的表达研究甚少。p53是迄今发现的重要抑癌基因，在多数肿瘤