胸腺细胞渐进性凋亡对大鼠脾脏淋巴细胞增殖功能的影响.DOCVIP

临床医学论文-胸腺细胞渐进性凋亡对大鼠脾脏淋巴细胞增殖功能的影响 ????????????????????? 作者：陈海英 陈建明 郑建盛? 【摘要】? 目的：研究胸腺内胸腺细胞电镜结构的渐进性、增龄性凋亡等形态学改变，探讨引起脾脏淋巴细胞增殖功能变化的可能机制。方法：光镜、电镜下观察胸腺主要结构的增龄性变化，测试凋亡细胞数。脾脏淋巴细胞增殖实验，脾脏组织流式细胞仪检测细胞凋亡率。并统计学分析。结果：在光镜下观察显示随着年龄增长，胸腺皮、髓质分界不清，胸腺细胞稀疏。电镜下胸腺细胞退化萎缩，出现大量凋亡小体。胸腺细胞凋亡百分率与成年年龄正相关（P0.05），脾脏内淋巴细胞在进入老年后才出现凋亡。成年组脾脏淋巴细胞增殖活性就开始出现明显增强（P0.05）。结论：随着鼠龄增大，而胸腺比脾脏内淋巴细胞更易于凋亡，胸腺细胞凋亡直接或间接促进脾脏淋巴细胞增殖功能。 【关键词】? 胸腺细胞；凋亡；脾脏；淋巴细胞转化；退化萎缩 ??? Abstract? Objective：To study the electron microscopic gradually morphologic change and apoptosis of the structual with age of? thymocytes in rat; and to explore its possible mechanism of proliferation of lymphocytes of rat’s spleens. Methods： The structure changes of the thymus were observed? by light and electron microscopic methods, testing apoptosis counts of the cells, the detection of spleens lymphocyte proliferation.Flow Cytometry Detection of Apotosis rate in Spleens was used to study the quantitatively and make? statistics analysis. Results： With age increasing, the line between cortex and medulla can’t be distinguished in the high power microscope. And thymocytes was few. Thymocyte was degenerative and involute. A great numer of apoptotic bodies were found. The thymocytes density ratio of cortex to medulla decreased is positive correlation with aging（P0.05）.The apoptotic lymphocyte of Spleens old age take place. Effective activation of in adult group spleens are much increased（P0.05）.Conclusion： With age increased,apoptosis? is? more easy with thymocytes? than that in spleens. Thymocytes apoptosis can directly or indirectly promote the proliferation power of spleens lymphocytes. ??? Key words? Thymocytes ;Apoptosis; Spleens; lymphocyte transformation; Invotion ??? 在衰老过程，机体的代谢和生理功能都有不同程度的改变，近年研究发现，免疫器官是机体对内外环境的适应和反应体系，从根本上参与了机体衰老的全过程。研究免疫系统在衰老过程中的变化具有重要的理论和临床意义。因此衰老对人体免疫影响的机制和防暑措施是当前国内外医学研究的重点内容。观察动物免疫器官的变化常选胸腺、脾脏，一般观察胸腺皮质厚度，胸腺皮质增厚、皮质 ??? 淋巴细胞增多［１］，提示机体免疫功能的提高。诸多学者用物理及化学等方法对胸腺结构及胸腺免疫功能的影响等不同领域做了相关研究。但任何研究都不能排除胸腺增龄变化对各项研究有广泛的影响［２］，本课题主要从形态学角度观察胸腺光镜、电