烧伤变性真皮基质可再生利用初步研究.pdfVIP

烧伤变性真皮基质可再生利用的初步研究 王晓川 姜笃银 山东大学第二医院急诊科 [摘要]目的 研制烧伤变性真皮基质(denatureddermalmatrix，DDM)，并对其进行初步评价 和检测，探讨DDM临床利用的可行性和生物学的机制。方法 采用Trypsin+TritonX-100法对大 鼠深Ⅱ度烫伤后1d、2d、3d皮肤进行脱细胞处理，制备DDM，即DDM-ld、DDM-2d、DDM-3d，行 大体、组织学和微生物学检测，并通过大鼠异体埋植实验，观察DDM的组织相容性和胶原变化情 况。结果 DDM质地柔软，有弹性，韧性较好；镜下无上皮结构和细胞存在，胶原排列紊乱；离 体微生物培养阴性；大鼠皮下埋植后伤口愈合良好,DDM-1d、DDM-2d组血管生长和炎症反应与对 照组无显著性差异，DDM-3d组炎症反应较强烈，大鼠存活率较其它各组低。结论 DDM没有明显 的免疫原性，生物相容性好，经过离体改造有望用于大面积烧伤的治疗。 [关键词]烧伤；变性真皮；细胞外基质;炎症反应 Preliminarystudyonrecyclingofthedenatureddermalmatrix WangXiao-chuan JiangDu-yin InstituteofTissueEngineeringofShandongUniversity,Departmentof Emergency, the SecondHospital,ShandongUniversity,Jinan250033,China;Correspondingauthor:JIANG Du-yin,Email:jdybs2@ [Abstract] Objective Todevelopamethodofproducingdenatureddermalmatrix(DDM)andits possiblepathogenesis.Toinvestigateitsavailabilityandbiologicalmechanismfor clinicalutilizationofDDM.MethodsTheskinofratspartialthicknessburnwound wastreatedwithtrypsin/tritonX-100andDDM(DDM-1d、 DDM-2d、 DDM-3d)wereobtained. TheresultingDDMwasexaminedmacroscopicallywithgrossobservation,histological assay,bacteriaculturetest,etc.ThentheDDMwereembeddedsubcutaneouslyinrats toinvestigatethedifferenceofthecompatibilityandcapacityforreconstructionof dermalcollagentissue.ResultsTheDDMwaspliable,elasticandductile.Thecells componentsweretakenoffcompletelyandcollagenfibersofDDMweredestructed.There wasnomicroorganisminDDM.Afterembeddedsubcutaneouslyinrats,thesatisfactory healingwaswell.Therewasnosignificantdifferenceinbloodvesselgrowthand inflammatoryreactionbetweenDDM-1、 2dgroupsandcontrolgroup,buttheinflammatory reactionintheDDM-3dgroupwasstrongerandthesurvivalratewaslower.Conclusion TheDDMhadverylowimunocompetenceandgoodbiocompatibility,soitwouldbe advantageoustoapplicationinthelargeareaburnsafrerreconstructedinvitro. [Keywords] Burns;Denatureddermis;Extracellularmat