细胞因子在慢性病毒性心肌炎心肌纤维作用及清心Ⅱ号的干预作用.DOCVIP

临床医学论文-细胞因子在慢性病毒性心肌炎心肌纤维作用及清心Ⅱ号的干预作用 ???????????? 作者：刘旺 窦丽萍 徐百鸿 王娟 程志清?? 【摘要】? ［目的］探讨TGFβ1在反复病毒感染致慢性病毒性心肌炎心肌纤维化对纤维连接蛋白及其受体整合素β1表达的影响与清心Ⅱ号干预作用。［方法］建立病毒性心肌炎慢性期心肌纤维化模型后小鼠随机分为模型组、卡托普利治疗组、清心Ⅱ号高、中、低治疗组,正常组和模型组给予生理盐水灌胃,治疗组分别给予卡托普利和清心Ⅱ号高中低剂量灌胃。疗程结束后分别采集心脏,应用RT-PCR技术检测CVB3-RNA；免疫组化技术检测TGFβ1和FN;免疫荧光染色-共激焦扫描分析整合素β1。［结果］造模后TGFβ1、整合素β1、FN明显增高，应用RT-PCR在心肌中可检测出病毒，清心Ⅱ号干预后TGFβ1、整合素β1、FN减低，各治疗组病毒减少。［结论］病毒、TGFβ1、整合素β1及FN在心肌纤维化形成中具有重要作用，清心Ⅱ号具有抗病毒、抗心肌纤维化作用。 【关键词】? 整合素β1；清心Ⅱ号；心肌炎；心肌纤维；TGFβ1;纤维连接蛋白;免疫组化；激光共聚焦 　　Abstract:［Objective］To study the effect of TGFβ1 on the expression of fibronectin and β1 integrin receptor in chronic viral myocarditis of myocardial fibrosis induced by repeated virus infection and the interventional effect of QingXin.［Methods］ Establish mice model of myocardial fibrosis in chronic stage of viralmyocarditis,then divide the mice model into model group，captopril group，QingXinⅡ high dose,medium dose and low dose groups.The normal group and model group are given saline through stomach perfusing，as well as the treated group are given respectively captopril and QingXinⅡ high,medium and low dose in the same way.After the treatment，hearts are collected，CVB3- RNA are detected by RT-PCR，TGFβ1 and FN are detected by immunohistochemical technique，integrinβ1 are analyzed by immunofluorescence staining-confocal laser scanning.［Results］ After having done the model，TGFβ1,integrinβ1,FN has increased obviously；virus can be detected in themyocardium by RT-PCR；after the intervention of QingXinⅡ，TGFβ1,integrinβ1,FN has decreased，virus has reduced in treated group.［Conclusion］ Virus，TGFβ1，integrinβ1 and FN have important effects on the formation ofmyocardial fibrosis；QingXinⅡ has the function of antivirus and anti-myocardial fibrosis.? 　　Key words:integrin β1；QingXin Ⅱ;myocarditis;myocardial fibrosis;TGFβ1;fibronectin；immuno-hischemical technique；confocal laser scanning ??? 病毒性心肌炎慢性期的主要病理特征为心脏间质纤维化,临床病理表现为心脏顺应性下降,僵硬度增加,进而引起舒张功能障碍,可发展为DCM,同时也是顽固性心律失常、慢性心功能不全难以逆转、猝死的重要原因之一。由于病毒的持续存在，心肌