功能纳米金粒子在重金属铅检测方面的研究论文2.docVIP

湖南化工职业技术学院 毕??业??论??文 功能纳米金粒子在重金属铅检测方面的研究 院系名称: 制药与生物工程系 分析1111 学生姓名: 舒婷婷 201103011121 指导老师: 王织云 3年11月 摘要 Pb2+检测方法十分重要。本论文针对纳米金在电解质溶液中易形成不可逆聚集的问题，研究了 DNA 分子修饰对纳米金溶胶稳定性的影响。紫外光谱的动力学特性曲线表明，经过与巯基相连的 DNA 修饰后，纳米金粒子能够在钠离子缓冲液中稳定存在，并研究了明胶等溶液对纳米金稳定性的影响。然后以“8-17”脱氧核酶为基础，合成了一种纳米金与脱氧核酶的聚集体并考察了聚集体的性质，并对Pb2+、Zn2+、Ca2+、Mg2+、Sn4+Fe3+等进行了检测，表明此种脱氧核酶聚集体对Pb2+有很好的选择性，为基于脱氧核酶的生物传感器的研究和应用奠定基础。 关键词：纳米金；修饰；脱氧核酶；Pb2+检测 Abstract Gold nanoparticles show promising applications in the field of molecular biologyand RD of biological sensors. It’s applications in biochemistry have been a hot topicthese years. Lead is very harmful to human and it’s very important to develop sensitiveand robust analytical approaches for sensing of Pb2+. aiming to overcome the irreversible aggregate of gold nanoparticles inelectrolyte solutions, the effect of DNA modification on the gold nanoparticles’ stability in presence of sodium ions was investigated, through the characterization ofUV-vis, TEM, and Zeta potential measurements. UV-vis spectra of kinetic propertiesfor the DNA modified gold nanoparticles reflect the high stability of the systemagainst the addition of sodium ions. Gelatin and other solutions were also studied onthe effect of stability of the gold nanoparticles. Secondly, based on the “8-17” DNAzyme, the aggregate of gold nanoparticlesand DNAzyme was synthesized and whose character was determined. In the presenceof Pb2+, the color of the aggregate turned from blue to red. Compared with Zn2+、Ca2+、Mg2+、Sn4+and Fe3+, it indicated that this DNAzyme aggregate had highlyselectivity on Pb2+, which is fundamental to the study and application of the biosensorbased on DNAzyme aggregate. Keywords: Gold nanoparticles, modification, DNAzyme, Pb2+detection 目录 第一章 绪论 1 1.1 纳米金的性质 1 1.2 纳米金的合成 2 1.2.1柠檬酸盐还原法 2 1.2.2 Brust-Schiffrin 的方法： 2 1.3纳米金的表征方法 3 1.4纳米金的应用 3 1.4.1纳米金用于免疫标记 4 1.4.2纳米金技