胆管癌端粒酶活性研究论文.pdfVIP

TelomeraseActivityinBileDuctCarcinoma Abstract Objective: Toinvestigatethetelomeraseactiviyt and significanceofclinicopathologyinbileductcarcinoma. Methods:Thetelomeraseactiviytof30casesbileduct carcinomaand23casesofadjacenttissuesweredetectedusing telomeric repeat amplification protocal--enzyme linked immunosorbentassaybasedonPCR(TRAP-ELISAassay). Simultaneously,8normalbileducttissueswereusedascontrols. Results:Twenyt-twoof30bileductcarcinomaandthreeof23 adjacenttissuespecimenswerepositivefortelomeraseactiviytwitha positiverateof73.3%and13.0%,allof8controlswasnegative. Themeanvalueoftelomeraseactiviytwas0.51,0.15and0.08, respectively(P0.001).Thetelomeraseactiviytvalueinthecases withlymphnodemetastases(0.67士0.35)washigherthanthat without(0.42士0.26)(P0.05).Astatisticallysignificantdifference wasfoundintelomeraseactivityvalueincarcinomasstage川andIV (0.66士0.33)comparedwithstageIand11 (0.37土0.24) (P0.01).Thevalueoftelomeraseactiviytinpoordifferentiation carcinoma(0.68士0.33)washigherthanthatinmoderateandhigh gradingcarcinoma(0.46士0.30)，howeverthedifferencewasnot statisticalsignificance(P0.05) Although,accordingtodifferent locationoftumors,thetelomeraseactiviytvaluewas difference(the uppersegmentofbileductwas:0.49,middle:0.65,low:0.42, respectively)，yettherewasnostatisticalsignificance(P0.05) Conclusions: Telomeraseactiviytplaysanimportantrolein bileductcarcinomaprogression.Thedetectionofatelomerase activiytmayserveasapowerfuladditionaltoolforthediagnosisof bileductcarcinomas. Keywords:Telomerase;Bileductcarcinoma;Polymerase chainreaction;Enzyme-linkedimmunosorbentassay 胆借启端粒阵活性研究 前 言 端粒 (`Ielomere)是典核细胞线性染色体末端的 段特殊区 域，山DNA及相关蛋白构)叼’1。端粒DNA山许多亩含G的串 联6-核一位酸重旬争列组成，在人类为((5-TTAGGG-3)。121，共 长150Kb.V在二十世纪三、四十年代，Mailer和McClintock发 现染色休末端端粒的存在对于染色体遗传功能的稳定和基因的 完整是不可缺少的。端粒主要具有如下功能:13.41(1)、维持染 色体结构的完整，防止其被核酸酶降解及染色体间相