He-Ne激光照射对淋巴结内巨噬细胞、淋巴细胞影响的实验研究.DOCVIP

临床医学论文-He-Ne激光照射对淋巴结内巨噬细胞、淋巴细胞影响的实验研究 ?????????作者;任明姬，姜丽萍，吴 岩，崔明玉 【摘要】? 目的? 探讨弱激光对机体免疫刺激作用的机理。方法? 用剂量63.7J/cm2的He-Ne激光照射小鼠神阙穴，照射时间为10天，每天照射5min，（1）采用台盼蓝活体注射显示巨噬细胞吞噬力试验方法；（2）采用吖啶橙（AO）显示核酸的荧光染色法分别观察小鼠肠系膜淋巴结内巨噬细胞的吞噬功能和淋巴细胞内核酸含量的影响。（3）观察淋巴结组织结构。结果? （1）照射组淋巴结内巨噬细胞吞噬功能较对照组显著增高，差异有非常显著性(P0.01)；（2）照射组淋巴细胞内核酸含量较对照组明显增多。（3）淋巴小结增多增大。结论? 适当剂量的He-Ne激光可激活淋巴结免疫功能，提高机体免疫力。??? ??? 【关键词】? 激光；巨噬细胞；? 淋巴细胞??? ??? An experimental study of? He- Ne? laser irradiation on the macrophages and lymphocytes in lymph node??????????? 【Abstract】? Objective? To research the effects of He-Ne laser irradiation on immunity of organism.Methods?? Shenque? acupoints of mice abdomen were irradiated by He-Ne laser at the dosages of 63.7J/cm2 for 10 days，5 minutes each day.Then following methods are taken：(1)Observing the phagocytosis of the macrophages in lymph node after trypan blue injecting the live mice.(2)Observing the changes of nucleic acid of lymphocytes with fluorescence microscope.(3) Observing? the? tissue structure? of? lymph node by HE stain.Results?? The four indexes：number of trypan granule，area of granule，ratio of granule area to cell area and granule integral optical density at irradiated group were higher than that of non-irradiated group (P0.01).The nucleic acid of irradiated groups were higher than non-irradiated group.In addition.the lymphoid nodule in the irradiated groups remarkable changes indicating their activation. Conclusion牋 He-Ne laser at appropriated dosages may activate lymph node and enhance the immunity of organism.牋?牋?【Key words】? laser;macrophage;? lymphocyte??? ??? 淋巴结是机体的主要周围淋巴器官，位于淋巴循环的通路上，其大小、结构及内含细胞成分的变化与机体的免疫功能状态密切相关。本文采用He-Ne激光照射小鼠神阙穴后，观察激光对肠系膜淋巴结组织结构和其内巨噬细胞吞噬力及淋巴细胞增殖能力的影响。旨在探讨低强度激光对机体免疫刺激作用机制，为临床应用提供实验依据。??? ??? 1? 材料与方法??? ??? 1.1? 材料? 昆明种小白鼠，体重18~22g，雌雄各半，随机分成对照组和照射组，采用上海产1000B(c)He-Ne激光器照射小鼠神阙穴（剪毛），照射功率25mW，照射剂量为63.7J/cm2，光斑直径0.3cm，照射时间为10天，每天照射5min。??? ??? 1.2? 方法? （1）取材前3日，每天往腹腔内注射0.5%的无菌台盼蓝0.3ml，第4日取肠系膜淋巴结。用10%福尔马林固定，常规法制成石蜡切片，用伊红淡然，将切片用