粉防己碱对高血压大鼠血管平滑肌细胞增殖的抑制作用.DOCVIP

临床医学论文-粉防己碱对高血压大鼠血管平滑肌细胞增殖的抑制作用 摘　要：探讨了粉防己碱（Tet）抑制培养的大鼠主动脉平滑肌细胞（AVSMC）增殖的作用，MTT法分析细胞增殖，［3H］TdR参入法分析细胞DNA合成.结果显示：①肾血管性高血压〔RH，二肾一夹（2K1C）术后18周〕大鼠AVSMC超微结构呈典型的合成细胞特征;②RH大鼠AVSMC具有更活跃的增殖倾向,血管紧张素Ⅱ(AngⅡ)和去甲肾上腺素(NE)刺激下指数增长期细胞数和在NE刺激下的AVSMC生长率均明显增高；③Tet（50mg.kg-1.d-1，po，2K1C术后9周始，连续9周）治疗组的AVSMC对NE和AngⅡ诱导的细胞增殖反应性和生长率较RH组明显降低；④对RH组和伪手术组大鼠的AVSMC，Tet（0.1～10μmol.L-1）体外给药可浓度依赖性地抑制NE或AngⅡ诱导的增殖和［3H］TdR参入.研究表明，RH大鼠AVSMC对NE和AngⅡ促增殖作用敏感性及反应性增高；Tet可降低其对NE和AngⅡ的反应性，抑制AVSMC增殖和DNA合成. 关键词：粉防己碱;高血压,肾血管性;肌,平滑,血管;细胞,培养的;细胞增殖 Depressive?effect?on?proliferation?of?vascular?smooth?muscle?cells?by?tetrandrine?in?hypertensive?rats LI?Qing-Ping（Department?of?Cardiovascular?Pharmacology,?Nanjing?Medical?University,?Nanjing?210029,?China）　 LU?Ze-An（Department?of?Cardiovascular?Pharmacology,?Nanjing?Medical?University,?Nanjing?210029,?China）　 RAO?Man-Ren（Department?of?Cardiovascular?Pharmacology,?Nanjing?Medical?University,?Nanjing?210029,?China）　 Abstract：To?analyse?the?effect?of?tetrandrine(Tet)?on?proliferation?of?aortic?vascular?smooth?muscle?cells?(?AVSMC),?AVSMC?were?isolated?and?cultured?from?sham-operated?rats(Sham),?renovascular?hypertensive?rats〔RHR,?18?weeks?after?two?kidney?one?clip(2K1C)?operation〕,?and?Tet?(50?mg.kg-1.d-1?po?for?9?weeks?from?week?9?after?2K1C?operation)treated?RHR.?The?proliferation?of?AVSMC?was?detected?by?MTT?method,?and?the?DNA?synthesis?was?evaluated?by?［3H］-thymidine?incorporation.?The?results?showed?that?①The?ultrastructure?of?aorta?suggested?that?AVSMC?in?RHR?had?transferred?from?contractile?phenotype?to?synthetic?phenotype;?②Compared?to?Sham,?AVSMC?from?RHR?showed?a?higher?proliferative?property?with?a?higher?cell?number?and?an?increased?growth?rate?stimulated?by?norepinephrine(NE)?or?angiotensinⅡ(AngⅡ);?③Compared?to?untreated?RHR,?AVSMC?from?Tet?treated?RHR?showed?a?reduced?reactivity?to?NE-?or?AngⅡ-stimulated?proliferation?and?growth?rate;?④Tet(0.1－10?μmol.L-1)?treated?in?vitro?induced?a?concentration-dependent?depression?in?［3H］?thymidine-incorporation?s