自配试剂免疫透射比浊比率法测定血清CIC.DOCVIP

临床医学论文-自配试剂免疫透射比浊比率法测定血清CIC 【摘要】??目的：寻求一种全自动仪测循环免疫复合物（CIC）的新方法，以取代传统方法繁琐的手工劳动和略显粗糙的定量结果。?方法：自配PEG和BBS试剂，以康宁560全自动生化仪结合AU560全自动生化仪设置程序最佳参数，免疫透射比浊终点法多点定标PEG和BBS，比率法计算CIC值，以百分回收试验和重复性试验确定其准确度和精密度，以对照试验与传统PEG沉淀法作对比分析。结果：仪测CIC最佳参数设置为：血清:试剂=1∶8，λ1340?nm，λ2450?nm，内源性读数时间20?Sec，总体反应时间288?Sec，总反应体积为180?μl，37℃。本方法与传统PEG沉淀法的回收率、日内和日间变异系数（CV%）分别为97%～110%、3.5%、4.3%和90%～122%、6.8%、9.7%。与传统PEG沉淀法相比，准确性差异无显著性（P＞0.05），两者具有较好的相关性（r=0.852）,精密度则比手工方法高。?结论：自配试剂上机检测CIC，其操作简便易行，且定量相对准确，重现性好，线性范围宽，完全可以取代繁琐的传统操作。? 【关键词】??免疫透射比浊法?比率法?循环免疫复合物 　　Serum?CIC?Determination?By?Immunological?Transmission?Turbidimetry?Ratio?with?Selfmix?Reagents ???? 　　Abstract:?Objective?To?look?for?a?new??fullautomatic?instrumental?method?for?Circulating?Immune?Complexes?（CIC）?determination,to?replace?the?tedious?handicraft?labor?of?traditional?method?and??gain?more?accurate??results.Methods?Try?to?mix??BBS?and??PEG?reagents?self,establish?the?best?procedure?parameters?with?Kangning?560?combine?with???AU560,immunological?transmission?turbidimetry??4?points?calibration??the?PEG?and??BBS,compute?the?CIC?value?with?ratio?method,then?judge?its?accuracy?and?degree?of?precision?by?percentage?recovery?ratio?study?and?repeatability?study,make?contrast?experiment?to?compare?with?traditional?PEG?precipitation?method.?Results?The??best?procedure?parameters?of?CIC?instrumental?method?were:Serum:Reagents=1:8,λ1340?nm,λ?2450?nm,endogenous?read?time?20?Sec,total?reaction?time?288?Sec,total?reaction?volume?180?ul,37?℃.?Percentage?recovery?ratio,inside?day?and?daytime?percentage?variation?coefficient(CV%)?of?this?method?and?traditional?PEG?precipitation?method?respectively?for?97%?～110%,3.5%,4.3%?and?90?%～122%,6.8%,9.7%.?Compared?with?traditional?PEG?precipitation?method,it?has?no?difference?in?accuracy（P＞0.05），the?two?have?good?correlation（r=0.852）,but?degree?of?precision?is?higher?than?handicraft?method.Conclusion?Serum?CIC?determination?by?immunological?transmission?turbidimetry??ratio??with??Selfmix?reagent,its?operation