三个苹果NBS基因的鉴定及其对外源生长物质的响应_柏素花.docVIP

178  植物生理学报 Plant Physiology Journal 2014, 50 (2): 178~184  三个苹果NBS基因的鉴定及其对外源生长物质的响应 柏素花1,2,宋霄1,张玉刚1,戴洪义1,* 青岛农业大学1园艺学院, 2生命科学学院, 山东省高校植物生物技术重点实验室, 山东青岛266109 摘要:为了探讨苹果NBS基因的生理作用,从苹果中鉴定了NBS家族的3个基因,分别命名为MdNBS、MdTIR-NBS1和 MdTIR-NBS-LRR1。这3个基因编码的蛋白质均含有NB-ARC结构域, MdTIR-NBS1和MdTIR-NBS-LRR1蛋白N端还有TIR 结构域, MdTIR-NBS-LRR1蛋白在C端含有LRR结构。荧光定量PCR分析表明,这3个不同类型的NBS基因的表达具有明显 不同的组织特异性,此外, MdNBS、MdTIR-NBS1和MdTIR-NBS-LRR1在‘嘎啦’苹果幼苗叶片中均受SA和MeJA诱导。ACC 处理不能提高MdNBS和MdTIR-NBS1基因的表达,但能诱导MdTIR-NBS-LRR1基因表达。结果表明, MdNBS、MdTIR-NBS1 和MdTIR-NBS-LRR1基因可能参与了苹果抗逆或抗病防御反应。 关键词:苹果; NBS基因;基因表达; SA; MeJA; ACC Identi?cation of Three Apple NBS Genes and Their Expression Responding to Exogenous Phytohormones BAI Su-Hua1,2, SONG Xiao1, ZHANG Yu-Gang1, DAI Hong-Yi1,* 1 College of Horticulture, 2Key Lab of Plant Biotechnology of Shandong Province, College of Life Sciences, Qingdao Agricultural University, Qingdao, Shandong 266109, China Abstract: To explore the physiological roles of apple NBS genes, three typical NBS genes were identi?ed from Malus×domestica and designated as MdNBS, MdTIR-NBS1 and MdTIR-NBS-LRR1. All the proteins encoded by the three genes contain a NB-ARC domain. In addition, both MdTIR-NBS1 and MdTIR-NBS-LRR1 proteins include a TIR motif in N terminus and MdTIR-NBS-LRR1 protein has a LRR motif in C terminus. The quantitative real-time PCR revealed that the expressions of these three NBS genes varied with different organs. MdNBS was highly expressed in functional leaves, MdTIR-NBS1 in buds and MdTIR-NBS-LRR1 in young leaves, respectively. Expression course analysis demonstrated that the expression of MdNBS, MdTIR-NBS1 and MdTIR-NBS-LRR1 in the young leaves of ‘Gala’ apple could be up-regulated by SA and MeJA. ACC treatment could not enhance the expression of MdNBS and MdTIR-NBS1, but could promote the expression of MdTIR- NBS-LRR1. Taken together, the results implied that the three genes might be involved in defense responses against biostress or abiostress in apple. Key words: Malus×dome