c在后处理抗大鼠肠缺血再灌注损伤细胞凋亡中的变化.pdfVIP

生理学报 Acta Physiologica Sinica, April 25, 2010, 62 (2): 143-148 143 研究论文 细胞色素 c 在后处理抗大鼠肠缺血 - 再灌注损伤细胞凋亡中的 变化 1,* 1 1 1 1 1 2 褚薇薇 ，聂蕾 ，和新盈 ，闫爱丽 ，周伊 ，吴庚利 ，王殿华 1 2 西安医学院基础部病理教研室，西安 710021 ； 昆明医学院天然药物药理重点实验室，昆明 650031 摘 要：本文旨在研究细胞色素c 在后处理抗大鼠肠缺血- 再灌注损伤细胞凋亡中的变化。将Sprague-Dawley 大鼠32 只随 机分为4 组(n =8) ：假手术(Sham) 组、缺血- 再灌注(I/R) 组、缺血预处理(IPC)组、缺血后处理(IPOST) 组。应用激光共聚 焦扫描显微镜检测各组大鼠肠黏膜细胞线粒体跨膜电位的变化。用Western blot 方法检测肠黏膜细胞线粒体内细胞色素c 及 caspase-3 表达的变化。末端脱氧核苷酸转移酶介导的dUTP 缺口末端标记法(TUNEL)和DNA 琼脂糖凝胶电泳方法检测大鼠 肠黏膜细胞凋亡发生情况。实验结果显示，与缺血- 再灌注组相比，缺血后处理组大鼠肠黏膜细胞线粒体跨膜电位显著升 高(P0.05) ，线粒体内细胞色素c 蛋白表达水平显著增加(P0.05) ，caspase-3 蛋白表达降低(P0.05) ，细胞凋亡率明显降低 (P0.05) 。缺血后处理组与缺血预处理组相比各项指标差异无统计学意义(P 0.05) 。上述结果提示缺血后处理可通过阻止线 粒体释放细胞色素c 抑制凋亡发生，减轻大鼠肠缺血- 再灌注损伤。 关键词：细胞色素c ；再灌注损伤；细胞凋亡 中图分类号：R6 57 .3 Change of cytochrome c in postconditioning attenuating ischemia-reperfusion- induced mucosal apoptosis in rat intestine 1,* 1 1 1 1 1 2 CHU Wei-Wei , NIE Lei , HE Xin-Ying , YAN Ai-Li , ZHOU Yi , WU Geng-Li , WANG Dian-Hua 1Department of Pathology, Xi’an Medical College, Xi’an 710021, China; 2 Yunnan Pharmacological Laboratory of Natural Products, Kunming Medical College, Kunming 650031, China Abstract: The present study aimed to investigate the change of cytochrome c in postconditioning-attenuated ischemia-reperfusion (I/R)-induced mucosal apoptosis in rat intestine compared with ischemic preconditioning (IPC). Using rat model of intestine I/R injury, male Sprague-Dawley rats weighing 220-250 g were divided into 4 groups which were Sham operation group, I/R group, IPC group and ischemic postconditioning (IPOST) group. In these groups, I/R procedure was performed by the occlusion of the superior mesenteric artery (SMA) for 45 min